黄独冻后黑暗培养愈伤组织再生苗同工酶分析  

Isoenzyme Analysis of Regenerated Plantlets from Dioscorea bulbifera L. Embryogenic Calli Cultured in Darkness after Cryopreservation

作　　者：柯维忠[1] 贺伟华[2] 计旭 卢湖娜 詹雪峰 刘文雅 王爱斌[1] Ke Weizhong;He Weihua;Ji Xu;Lu Huna;Zhan Xuefeng;Liu Wenya;Wang Aibin(College of Life Sciences, Shangrao Normal University, Shangrao, 334001;Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang, 330096)

机构地区：[1]上饶师范学院生命科学学院,上饶334001 [2]江西省科学院微生物研究所,南昌330096

出　　处：《分子植物育种》2018年第7期2266-2273,共8页Molecular Plant Breeding

基　　金：国家自然科学基金(31360072)资助

摘　　要：本研究对黄独冻后黑暗培养胚性愈伤组织及其再生植株的同工酶进行比较分析。结果表明,与黄独冻后光周期培养胚性愈伤组织相比,黄独冻后黑暗培养胚性愈伤组织及其再生植株的CAT、SOD、POD和淀粉酶同工酶酶谱条带数不变,淀粉酶同工酶酶谱亮度和酶谱面积无变化,但CAT、SOD、POD和淀粉酶同工酶酶谱的亮度明显增加,酶谱面积显著增大;与黄独带芽茎段常温继代培养再生植株相比,黄独冻后黑暗培养胚性愈伤组织再生植株的CAT、SOD、POD和淀粉酶同工酶酶谱条带数也不变,淀粉酶同工酶酶谱亮度和酶谱面积也无变化,但CAT、SOD、POD和淀粉酶同工酶酶谱的亮度也明显增加,酶谱面积也显著增大。因此,冻后黑暗培养可以提高黄独胚性愈伤组织的抗氧化机能,且其超低温保存能保证黄独的遗传稳定性。This paper comparatively analyzed the isoenzymes ofDioscorea bulbifera embryogenic ealli cultured in darkness after cryopreservation and its regenerated plantlets. The results showed that compared with embryogenic calli cultured indirectly in photoperiod after cryopreservation, the isozyme band number of CAT, SOD, POD and amylase of embryogenic calli cultured in darkness after cryopreservation was unchanged, and amylase isozyme zymogram and brightness area also did not change, but the brightness of the CAT, SOD, POD and amylase isozyrne and their enzyme spectrum area significantly increased; compared with plantlets regenerated from stems with a bud at room temperature, the isozyme band number of CAT, SOD, POD and amylase of plantlets regenerated from embryogenic calli cultured in darkness after cryopreservation also was unchanged, and amylase isozyme zymogram and brightness area did not change as well, while the brightness of the CAT, SOD, POD and amylase isozyme and their enzyme spectrum area significantly increased. Therefore, dark culture after cryopreser- vation could improve antioxidant function of their embryogenic calli, and cryopreservation could ensure the genetic stability ofD. bulbifera.

关 键 词：黄独 冻后黑暗培养 胚性愈伤组织 再生植株 同工酶 

分 类 号：S567.239[农业科学—中草药栽培]