前列腺素E_1对高糖培养小鼠肾足细胞整合素及整合素连接激酶影响的研究  

Effect of Prostaglandin E_1 on expression of integrin β1 and integrin-linked kinase in mouse podocyte cultured in high glucose

作　　者：张扬[1] 王瑞荣[1] 于龙丽[1] 徐晓娜[2] ZHANG Yang;WANG Ruirong;YU Longli(Department of Nephrology , Qingdao Municipal Hospital ,Qingdao 266011, China)

机构地区：[1]青岛市市立医院肾内科,266011 [2]青岛市市立医院中心实验室,266011

出　　处：《中国糖尿病杂志》2018年第4期332-337,共6页Chinese Journal of Diabetes

摘　　要：目的研究前列腺素E_1(PGE_1)对高糖培养小鼠足细胞整合素及整合素连接激酶(ILK)的影响。方法体外培养和分化小鼠肾小球足细胞,按培养条件不同分为:5.6 mmol/L葡萄糖组(Con),5.6 mmol/L葡萄糖+24.4 mmol/L甘露醇组(5.6 mmol/L+24.4 mmol/L),15 mmol/L葡萄糖组(15mmol/L),30 mmol/L葡萄糖组(30 mmol/L)。每个培养条件分别以0、1、10、20 ng/ml的PGE_1进行干预,24 h后收集足细胞。Western blot检测整合素β1(Integrinβ1)、ILK、α-平滑肌肌动蛋白(α-SMA)的表达情况。结果依次增高葡萄糖浓度下足细胞Integrinβ1蛋白表达相对量依次降低,ILK、α-SMA蛋白表达相对量依次升高。30 mmol/L组足细胞Integrinβ1、ILK、α-SMA蛋白表达相对量为(0.32±0.04)、(1.20±0.02)、(1.24±0.02),与Con组比较差异有统计学意义(P<0.05);添加20ng/ml的PGE_1对高糖引起的足细胞上述蛋白异常表达有逆转作用,Integrinβ_1、ILK、α-SMA蛋白相对表达量分别为(0.91±0.02)、(0.26±0.02)、(0.42±0.02),较各组中PGE_1=0时差异有统计学意义(P<0.05)。结论 PGE_1对高糖环境中足细胞ILK、α-SMA蛋白表达上调具有抑制作用,对Integrinβ1蛋白表达具有上调作用,对高糖引起的足细胞转分化有抑制作用。Objective To investigate the effect of Prostaglandin E1 （PGE1） on expression of integrin β1 and integrin-linked kinase in mouse podocyte cultured in high glucose. Methods Mouse podocytes were cultured in vitro culture and induced differentiation. Then the podocytes were randomly divided into four groups according to the culture conditions： 5.6 mmol/L glucose control group, 5.6 mmol/L glucose and 24.4 mmol/L mannitol group, 15,30 mmol/L glucose group. Each group was intervened with 0,1,10, 20 ng/ml PGE1 for 24 h. The expression of Integrin β1, Integrin-linked Kinase （ILK）, and α-smoothmuscle actin （α-SMA）was detected by Western blot. Results The relative expression of integrin β1 was decreased and the relative expression of ILK and α-SMA protein was increased in different glucose concentration. The relative expression of Integrinβ1, ILK and α-SMA protein in 30 mmol/L glucose group [（0.32±0.04）, （1.20±0. 02）, （1.24±0. 02）] was significantly different from normal control group （P〈 0.05）. The adding of 20 ng/ml PGE1 had a reversal effect on the abnormal expression of podocytes induced by high glucose. The relative expression levels of Integrin β1, ILK and α-SMA were （0.91± 0. 02）, （0. 26±0. 02） and （0. 42±0. 02） respectively, which was significantly different from that in PGE1 =0 ng/ml groups （P〈0. 05）. Conclusion PGE1 inhibited the high expression of ILK and α-SMA,and increasedthe expression of Integrin β1 in podocytes. PGE1 could inhibit podocyte epithelial-mesenchymal transition induced by high glucose.

关 键 词：前列腺素E1 高糖 足细胞 整合素Β1 整合素连接激酶 Α-平滑肌肌动蛋白 

分 类 号：R587.2[医药卫生—内分泌]