冰川水在人真皮淋巴管内皮细胞体外培养模型上的护肤功效研究  

Skin protection efficacy evaluation of glacier water based on in vitro culture of human dermal lymphatic endothelial cells

作　　者：蒋丹丹 李慧 姜山山 DOS SANTOS Morgan JIANG Dan - dan;LI Hui;JIANG Shan- shan;DOS SANTOS Morgan(R＆D Center, JAlA （Group） Co. , Ltd. , Shanghai 200233, Chin)

机构地区：[1]伽蓝(集团)股份有限公司研发中心,上海200233

出　　处：《日用化学工业》2018年第4期216-220,共5页China Surfactant Detergent & Cosmetics

摘　　要：采用两步免疫磁化分离方法,从正常人皮肤组织中分离并富集出真皮淋巴管内皮细胞,用于构建体外培养模型,经流式细胞仪检测细胞纯度指标即Podoplanin阳性细胞比例为95.7%。为评估喜马拉雅冰川水对真皮毛细淋巴管的功效,利用人真皮淋巴管内皮细胞构建毛细淋巴管通透性模型进行检测,结果显示冰川水处理后的淋巴内皮细胞层通透性是对照组的49.3%±17.0%(P<0.05);利用细胞爬片免疫荧光染色方法进行分析,结果显示冰川水处理组在胞间连接蛋白VE-Cadherin和ZO-1的表达量上均有显著提高(P<0.05),因此冰川水具有促进真皮毛细淋巴管稳定性的护肤活性。Human dermal lymphatic capillaries were reported to have impact on skin homeostasis in recent researches. Human dermal lymphatic endothelial cells（ HDLEC） were extracted from normal human skin biopsy and enriched with a two-step magnetic microbeads-based separation method. The proportion of Podoplanin-positive cells in HDLEC culture was evaluated by flow cytometry as 95. 7%. To evaluate the skin care efficacy of Himalaya glacier water,an in vitro lymphatic capillary permeability test model was reconstructed based on HDLEC culture,and the test result shows the relative permeability of HDLEC monolayer turned to 49. 3% ± 17. 0%（ P〈 0. 05） after the treatment of glacier water compared with that from the untreated control. In the meantime,immuno-cytochemistry staining targeting endothelial cell-cell junction markers show relative expressions of VE-Cadherin and ZO-1 in glacier water-treated HDLEC are significantly up-regulated than the untreated control（ P 〈0. 05）. Therefore,Himalaya glacier water is proved to be active in reinforcing the stability of dermal lymphatic capillaries to maintain skin homeostasis.

关 键 词：护肤 真皮 淋巴管内皮细胞 免疫磁化分离 毛细管通透性 

分 类 号：TQ658[化学工程—精细化工]