HPLC测定朝鲜白头翁中常春藤皂苷C的含量  

Determination of Hederacoside C in Roots of Pulsatilla cernua by HPLC

作　　者：任乔森 王海洋[1] 翟宏宇[1] 赵琳琳[1] 李秀芬[1] 唐秋竹[1] REN Qiao-sen;WANG Hai-yang;ZHAI Hong-yu;ZHAO Lin-lin;LI Xiu-fen;TANG Qiu-zhu(Jilin Institute for Drug Control, Changchun 130033, China)

机构地区：[1]吉林省药品检验所,吉林长春130033

出　　处：《中国现代中药》2018年第1期42-45,共4页Modern Chinese Medicine

摘　　要：目的:建立高效液相色谱法测定朝鲜白头翁中专属性成分常春藤皂苷C的定量分析方法。方法:采用Thermo Hypersil BDS C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(28∶72)作为流动相进行洗脱,流速:0.8 m L·min^(-1),柱温:35℃,检测波长:201 nm。结果:常春藤皂苷C在0.220~2.203μg(r=0.999 9)线性关系良好,回收率为97.25%。结论:该方法专属性强,结果准确可靠,重现性好,可用于朝鲜白头翁的质量控制。Objective： To establish a method for the content determination of hederacoside C in roots of PulsatiUa cernua by HPLC. Methods： The determination was performed on Thermo Hypersil BDS C18 column （250 mm × 4. 6 mm, 5 μm）. The mobile phase was acetonitrile and water （28 ..72） with the flow rate of 0. 8 mL. min-1. The temperature was 35 ℃ and detection wavelength was 201 nm. Results： the linear ranges of hederacoside C was 0. 220-2. 203 μg （ r = 0. 999 9）. Conclusion： The method is highly specific, accurate and reliable and has good repeatability. It is suitable for the quality control of the roots of P. cernua.

关 键 词：高效液相色谱法 朝鲜白头翁 常春藤皂苷C 含量测定 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]