紫外分光光度法测定贵州马兰总蒽醌含量  

Determination of total anthraquinone in Kalimeris indica by ultraviolet-visible spectrophotometry

作　　者：谢浪 李嘉 陈晓兰 邓显仪 王洁洁 XIE Lang;LI Jia;CHEN Xiaolan;DENG Xianyi;WANG Jiejie(College of Pharmacy, Guiyang University of Chinese Medicine, Guiyang 550002, Chin)

机构地区：[1]贵阳中医学院药学院,贵州贵阳550002

出　　处：《贵州科学》2018年第2期16-20,共5页Guizhou Science

基　　金：贵州省科技联合基金(黔科合LH字[2015]7293号);贵州省教育厅青年人才项目(黔科合KY[2016]184号)

摘　　要：目的:测定贵州马兰中总蒽醌的含量。方法:将1,8-二羟基蒽醌对照品溶液和马兰样品溶液于200～400 nm波长范围内进行扫描,确定检测波长为223 nm,建立紫外分光光度计测定贵州马兰中总蒽醌含量。结果:1,8-二羟基蒽醌对照品溶液浓度在2.2～6.05μg/m L的范围内,其浓度与吸光度呈良好的线性关系,C=169.43A-0.1899(r=0.9996);样品加样回收率为98.31%,RSD为2.82%(n=6);贵州马兰中总蒽醌含量为3.146mg/g,RSD为1.60%(n=5)。结论:建立的紫外分光光度法操作简便,重复性良好,可用于马兰中总蒽醌的含量测定;该法可为马兰质量控制和制定质量标准提供参考。In this study we determined the content of total anthraquinone in Kalimeris indica in Guizhou. We scanned the 1,8-dihydroxy anthraquinone standard solution and the sample solution at the wavelength of 200 nm to400 nm,and determined the detection wavelength of 223 nm. Then ultraviolet-visible spectrometer was used to determine the content of total anthraquinone in Kalimeris indica. The results showed that,within the concentration range of 2. 2 μg/m L to 6. 05 μg/m L,the concentration of 1,8-dihydroxy anthraquinone standard solution was in good linear relationship with the absorbance,C = 169. 43 A-0. 1899（ r = 0. 9996）. The recovery rate was 98. 31%,and the RSD was 2. 82%（ n = 6）. The content of total anthraquinone in Kalimeris indica was 3. 146 mg/g,and the RSD was 1. 60%（ n = 5）. The established ultraviolet-visible spectrophotometry method has the advantages of easy operation and good repeatability,and can be used in the determination of total anthraquinone in Kalimeris indica.This method can provide reference for the quality control and quality standard of Kalimeris indica.

关 键 词：马兰 含量测定 紫外分光光度法 总蒽醌 

分 类 号：R282.4[医药卫生—中药学]