碳离子束辐射诱变拟南芥突变体盐胁迫研究  

Research on Salt Stress Tolerance Mutant of Arabidopsis thaliana Irradiated by Carbon Ion Beam

作　　者：骆善伟[1,2] 杜艳 余丽霞[1] 陈玉泽[1,3] 崔涛[1,2] 冯慧 穆金虎[1,2] 李文建 周利斌 LUO Shanwei;DU Yan;YU Lixia;CHEN Yuze;CUI Tao;FENG Hui;MU Jinhu;LI Wenjian;ZHOU Libin(Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;University of Chinese Academy of Sciences, Beijing 100049, China;College of Life Science and Technology, Gansu Agricultural University, Lanzhou 703370, China)

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]中国科学院大学,北京100049 [3]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《原子核物理评论》2018年第1期94-99,共6页Nuclear Physics Review

基　　金：国家自然科学基金资助项目(11275171);中国科学院战略性科技先导专项项目(XDA08040111);中国科学院西部之光人才培养引进计划资助项目(29Y506020);中国科学院青年创新促进会(2015337)

摘　　要：非生物逆境胁迫是影响植物生长的重要因素之一,植物耐盐研究是抗逆研究的一个重要组成部分。利用兰州重离子研究装置(HIRFL)产生能量为43.3 MeV/u的碳离子束辐射诱变拟南芥干种子,获得了稳定突变体#197,通过生理指标测定和实时荧光定量PCR技术对该突变材料进行盐胁迫相关研究,结果表明:#197在盐胁迫条件下萌发速率低于野生型,幼苗的绿胚率较野生型更高。基因表达方面,#197受到高盐胁迫后,P5CS1基因迅速转录,且表达水平显著高于野生型,而脯氨酸降解相关基因Pro DH的表达量#197略低于野生型,该表达模式有利于脯氨酸的积累,从而缓解了#197受到的胁迫压力,使得DREB2A和RD29A的表达量低于同期野生型。Abiotic stress can reduce the growth of plant heavily, and the research of salt tolerance of plant mutant is valuable to the study plant stress resistance. Dry seeds of Arabidopsis thaliana were irradiated by carbon ion beams with energy of 43.3 MeV/u provided by Heavy Ion Research Facility in Lanzhou（HIRFL）. One stable mutant #197 was obtained from the progenies of the irradiated populations, the study of tolerance to salt stress of this mutant were carried out through physiological and gene transcription anlaysis. The results demonstrated：mutant #197 shown a relatively lower germination rate than wild type in salt stress conditions, meanwhile, the mutant acquired a higher ratio of green embryo than the wild type. In the molecular level, Na Cl with high concentrations prompted the transcription of P 5 CS1 gene rapidly both in #197 muatant and the wide type, and the expression level in mutant was significantly higher than wild type. The expression of P ro DH, related to proline degradation, in mutant #197 was slightly less than the wide type. Such expression patterns improved the accumulation of proline, and protect the #197 mutant from salt stress, which may lead to a lower expression of DREB2 A and RD29 A in mutant compared with wide type.

关 键 词：碳离子束 拟南芥 突变体 耐盐 脯氨酸 

分 类 号：Q691.5[生物学—生物物理学] Q947.9