牛磺熊去氧胆酸诱导牙髓干细胞的成骨分化实验研究  

Taursodeoxycholic Acid Induced the Osteogentic Differentiation Ability of Dental Pulp Stem Cells

作　　者：陈福扬[1] 孙艳艳 谭金娣 于易秀 刘明月[1] 史欣[1] 胡伟平[1] CHEN Fu-yang;SUN Yan-yan;TAN Jin-di;YU Yi-xiu;LIU Ming-yue;SHI Xin;HU Wei-ping(Department of Prosthodontics,the Second Affliated Hospital of Harbin Medical University,Harbin150086,Heilongjiang,China;Department of Prosthodontics,Xuzhou Stomatology Hospital,Xuzhou 221000,Jiangsu,China)

机构地区：[1]哈尔滨医科大学附属第二医院口腔修复科,黑龙江哈尔滨150086 [2]徐州市口腔医院修复科,江苏徐州221000

出　　处：《中国美容医学》2018年第4期97-101,共5页Chinese Journal of Aesthetic Medicine

基　　金：黑龙江省自然科学基金(H2013101)

摘　　要：目的:研究牛磺熊去氧胆酸(Tauroursodeoxycholic acid,TUDCA)对牙髓干细胞(Dental pulp stem cells,DPSCs)增殖及成骨向分化的作用。方法:用不同质量浓度(0、10、100、30OnM)的TUDCA培养DPSCs,利用甲基噻唑基四唑(Methylthiazolylterrazolium,MTT)法计数检测不同浓度TUDCA对细胞增殖的影响;7d后测定不同浓度TUDCA诱导后的碱性磷酸酶(Alkaline phosphatase,ALP)活性;倒置相差显微镜下观察诱导后的DPSCs形态变化;应用茜素红染色法检测矿化结节的形成。结果:MTT法及ALP活性测定实验表明,随着TUDCA浓度的增加,ALP活性增强,细胞增殖率升高。茜素红染色显示TUDCA诱导培养28d后出现矿化结节。结论:TUDCA可促进DPSC增殖和成骨分化。Objective To study the effect of tauroursodeoxycholic acid （TUDCA） on the proliferation and osteogenic differentiation in dental pulp stem cells （DPSCs）. Methods Methylthiazolyltetrazolium （MTT） assay was used to evaluate the proliferation rate of DPSCs induced by different concentrations of TUDCA（0,10,100,300nM）. The activity of alkaline phosphatase （ALP） after induced by different concentration of TUDCA was measured. We observed the morphologica1 changes of DPSCs by the inverted phase contrast microscope, detected the mineralize nodules stained by Alizarin red. Results The results of MTT and ALP activity measurement showed that ALP activity increased and cell proliferation rate arise with the increase of concentration of TUDCA. Alizarin red staining showed that mineralized nodules have formed after induced by TUDCA for 28 day. Conclusion TUDCA can promote the proliferation and osteogenic differentiation of DPSCs.

关 键 词：牙髓干细胞 牛磺熊去氧胆酸 细胞增殖 成骨分化 新型生物材料 组织工程 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]