万寿菊-烟草套作体系中不同生长期烟草根际土壤微生物多样性研究  

Microbial Diversity of Tobacco Rhizosphere Soil in Different Growth Stages of Marigold-tobacco Intercropping System

作　　者：夏体渊[1] 陈泽斌[1] 苏源 刘佳妮[1] 余磊 王定康[1] 徐胜光 XIA Ti-yuan;CHEN Ze-bin;SU Yuan;LIU Jia-ni;YU Lei;WANG Ding-kang;XU Sheng-guang(Agriculture College, Kunming University, Yunnan Kunming 650214 , China;Key Laboratory of Special Biological Resource Devel-opment and Utilization of Universities in Yunnan Province, Yunnan Kunming 650214, China;Engineering Research Center for Charac-teristic Agriculture of Yunnan Province, Yunnan Kunming 650214 , China;Engineering Research Center for Biochar of High Education in Yunnan Province, Yunnan Kunming 650214, China)

机构地区：[1]昆明学院农学院,云南昆明650214 [2]云南省高校特色生物资源开发与利用重点实验室,云南昆明650214 [3]云南省都市特色农业工程技术研究中心,云南昆明650214 [4]云南省高校生物炭工程研究中心,云南昆明650214

出　　处：《西南农业学报》2018年第4期680-686,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(41361056);昆明学院人才引进项目(YJL14005);昆明学院决策咨询项目(2017JC11);云南省高校优势特色重点学科(生态学)建设项目(05000511311);云南省特色生物资源开发与利用重点实验室开放基金项目(GXKZ201716);云南省地方本科高校基础研究联合专项(2017FH001-041)

摘　　要：【目的】为揭示万寿菊与烟草套作体系中不同生长期烟草根际微生物多样性。【方法】采用Illumina Mi Seq高通量测序技术测定烟草团棵期、旺长期、现蕾期和成熟期根际土样的16S-V4区及ITS1区序列。利用Flash软件拼接、Qiime软件过滤原始序列,整理各样品有效序列和分类操作单元,对物种多样性进行分析。【结果】本研究得到260 578条细菌序列,划分为14 360个OTUs;得到189 716条真菌序列,划分为3288个OTUs。现蕾期细菌香农指数最大,且套作处理香农指数高于单作,说明现蕾期细菌种类最丰富,套作可提高细菌多样性;对于真菌而言,套作处理香农指数呈现先升高后降低最终又升高的趋势,与单作变化趋势相同,并且在成熟期达到最大值,说明套作可增加成熟期的真菌种类多样性。【结论】套作降低了旺长期食碱菌属(Alcanivorax)和海杆菌属(Marinobacter)的丰度,且增加了团棵期锥盖伞属(Conocybe)的丰度,降低了成熟期Microidium属的丰度,改变了不同生长期的真菌群落组成结构。【Objective】The present paper was conducted to reveal the diversity of rhizosphere microorganisms of marigold and tobacco intercropping system in different growth stages.【Method】The sequences of bacterial 16 S-V4 region and the fungal ITS1 region of the rhizosphere samples at the resettling,vigorously growing,squaring and maturation stage were measured using Illumina Mi Seq high-throughput sequencing technique,then the sequences of different samples and operational taxonomic units were arranged by stitching with flash and filtering with Qiime for the analysis of diversity of species and difference of distribution.【Result】260 578 effective bacterial sequences and 14 360 OTUs,189 716 effective fungal sequences and 3288 OTUs were obtained.The Shannon index was the highest at the squaring stage,and interplanting had higher Shannon index than monoculture,indicating that there was a diversity of bacterial species at the squaring stage and intercropping would increase the differences among the bacterial species.For fungi,Shannon index with the intercropping increased first and then decreased eventually,monoculture had the same change trend,and reached the maximum at maturity.【Conclusion】Intercropping significantly reduced the amount of Alcanivorax and Marinobacter at the vigorously growing stage,intercropping increased the amount of Conocybe at the resettling stage while reducing the amount of Microidium at the maturation stage,which changed the composition of fungal community at different growth stages.

关 键 词：烟草 万寿菊 套作 根际土壤 不同生长期 

分 类 号：S572[农业科学—烟草工业]