全基因组预测里氏木霉QM6a的碳水化合物活性酶类蛋白  

Prediction for CAZymes from Trichoderma reesei QM6a Genome

作　　者：韩长志 祝友朋 许僖 HAN Chang-zhi;ZHU You-peng;XU Xi(College of Biodiversity Conservation and Utilization/The Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, South-west Forestry University, Yunnan Kunming 650224 , Chin)

机构地区：[1]西南林业大学生物多样性保护与利用学院/云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《西南农业学报》2018年第4期705-710,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目"钩状木霉与枯草芽孢杆菌对核桃炭疽病的协同生防作用机制研究"(31560211);云南省森林灾害预警与控制重点实验室开放基金项目"钩状木霉与枯草芽孢杆菌对核桃炭疽病的协同控制技术研究"(ZK150004);云南省优势特色重点学科生物学一级学科建设项目(50097505);云南省高校林下生物资源保护及利用科技创新团队(2014015)

摘　　要：【目的】里氏木霉QM6a是一株产纤维素酶的重要工业菌株,明确该菌中碳水化合物活性酶类蛋白(CAZymes),为进一步开展其功能研究打下坚实的理论基础。【方法】基于前期所获得的356个分泌蛋白数据,利用CAT预测程序对其CAZymes进行分析。【结果】里氏木霉QM6a中含有201个CAZymes,可以分为主要类别、复合类别,主要类涉及93个GHs和52个CBMs以及15个AAs、14个CEs、5个PLs、4个GTs;复合类别则涉及15个GH/CBMs和1个AA/CBMs以及2个CE/CBMs。【结论】通过上述生物信息学分析方法有效地实现了里氏木霉碳水化合物活性酶蛋白的预测。【Objective】Trichoderma reesei QM6a is an important industrial production strains for cellulase production.To identify the carbohydrate-active enzymes（CAZymes） protein from T.reesei and clear its characteristic can lay a solid theoretical foundation for further study of its function.【Method】Based on the 356 secreted proteins obtained from preliminary study,CAZymes protein was predicted by using CAT forecasting procedures.【Result】201 CAZymes proteins were found in T.reesei,which were divided into major categories and composite category,and the former included 93 GHs,52 CBMs and 15 AAs,14 CEs,5 PLs,4 GTs; the latter included 15 GH/CBMs,1 AA/CBMs,2 CE/CBMs.【Conclusion】Through the above bioinformatics analysis,the predicted CAZymes proteins can effectively achieve in T.reesei.

关 键 词：里氏木霉 碳水化合物酶类 预测程序 生物信息学 

分 类 号：S436[农业科学—农业昆虫与害虫防治]