湖南桃江病圃92个稻瘟病菌株遗传多样性分析  

Analysis of Genetic Diversity of 92 Magnaporthe oryzae in Rice Blast Nursery at Taojiang,Hunan

作　　者：周瑚 任佐华[1,2] 刘品克 张译允 王恒沪[1] 刘二明 ZHOU Hu;REN Zuo-hua;LIU Pin-ke;ZHANG Yi-yun;WANG Heng-hu;LIU Er-ming(College of Plant Protection, Hunan Agricultural University, Hunan Changsha 410128 , China;Hunan Provincial Key Laboratory for Bi-ology and Control of Plant Diseases and Insect Pests, Hunan Changsha 410128 , China;Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Hunan Changsha 410128 , China)

机构地区：[1]湖南农业大学植物保护学院,湖南长沙410128 [2]植物病虫害生物学与防控湖南省重点实验室,湖南长沙410128 [3]南方粮油作物协同创新中心,湖南长沙410128

出　　处：《西南农业学报》2018年第4期725-730,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0300700);公益性行业(农业)科研专项(201203014);湖南省"十二五"重点学科(0904)

摘　　要：【目的】利用抗瘟水稻品种是防治稻瘟病最有效措施,了解稻瘟病菌遗传多样性是抗病品种合理布局的重要基础。【方法】本研究于2015年在湖南桃江病圃采集水稻品种"丽江新团黑谷"(LTH)稻瘟菌病样,用单孢分离法分离稻瘟病菌单孢并纯化获得92个单孢菌株,利用14对SSR引物对其进行遗传多样性分析。【结果】在0.19相似水平,92个供试菌株可被划分在同一遗传宗谱内;在0.70相似水平,92个菌株可被划分成26个遗传宗谱,优势宗谱XIX内含有17个菌株,占总菌株数的18.48%,宗谱Ⅳ、XVIII中分布包含10、9个菌株,分别占总菌株数的10.87%、9.78%;其他23个宗谱内各含1~5个菌株。【结论】综合表明,湖南桃江病圃的稻瘟病菌遗传差异较大,个别菌株亲缘关系较远,遗传结构比较复杂。【Objective】Utilization of resistant cultivars is the most effective method to control rice blast,and it is an important basis of rational distribution of resistant varieties to clarify the genetic diversity of Magnaporthe oryzae.【Method】The genetic diversity of 92 isolates of M.oryzae from the susceptibe variety Lijiangxintuanheigu（LTH） in rice blast nursery at Taojiang,Hunan in 2015,the genetic diversity of 92 isolates was analyzed by 14 pairs based on SSR primers.【Result】All of the 92 tested isolates were classified into the same genetic lineages at the 0.19 similar level; these isolates were classified into 26 genetic lineages at the 0.70 similar level,the dominant lineage XIX contains17 isolates,18.48 of the total number of isolates.There are 23 lineages within the genealogy contains the amount between 1 and 5 strains.【Conclusion】From the atlas of clustering analysis separation of taojiang in Hunan disease nursery of the 92 genetic differences of rice blast fungus,genetic structure is more complex,more distant genetic relationship of individual strains.

关 键 词：稻瘟病菌 SSR 遗传多样性 宗谱 

分 类 号：S434.111.41[农业科学—农业昆虫与害虫防治]