青杨天牛触角味觉受体基因的鉴定  

Identification of Saperda populnea Antennal Gustatory Receptor Gene

作　　者：王佳同 张健[2] 程彬[2] 王寅亮[1] 陈琪[1] 任炳忠[1] WANG Jia-tong, ZHANG Jian, CHENG Bin(1. School of Life Sciences, Northeast Normal University, Changchun,Jilin 130024; 2. Jilin City Academy of Forestry, Changchun, Jilin 130033)

机构地区：[1]东北师范大学生命科学学院,吉林长春130024 [2]吉林省林业科学研究院,吉林长春130033

出　　处：《安徽农业科学》2018年第13期111-113,121,共4页Journal of Anhui Agricultural Sciences

基　　金：林业公益性行业科研专项(201404413);吉林省公益性科研院所基本科研业务费项目(GY-2018-10)

摘　　要：[目的]鉴定青杨天牛(Saperda populnea)触角味觉受体基因的种类。[方法]根据青杨天牛触角总RNA的转录组测序结果,结合生物信息学分析方法对其味觉基因进行鉴定,并采用RT-PCR技术测定味觉受体基因在不同组织中的表达量。[结果]共鉴定出青杨天牛味觉受体基因11种,其中4种全长味觉受体基因(SpopGR1、SpopGR3、SpopGR4、SpopGR5)在青杨天牛雌雄触角均有表达,除SpopGR5,另外3个基因均在足表达,SpopGR1和SpopGR4在雌触角、雄触角、头、胸、腹、足和翅这7个部位均有表达,但表达量偏低。[结论]通过与果蝇味觉受体基因建树分析,推测SpopGR7、SpopGR9、SpopGR10可能属于苦味受体家族,行使对苦味的感知功能。该研究有助于在分子水平上加深对昆虫味觉感知系统的理解,为以后采用化学生态手段对青杨天牛进行调控提供依据。[Objective]To identify the types of gustatory receptor genes of Saperda populnea. [Method]To identify the types of gustatory receptor genes based on the transcriptomes information of total RNA of antennae of S. populnea connected with bioinformatics analysis method. Expressions of gustatory receptors of S. populnea were measured with RT-PCR technique. [Result]11 types of gustatory receptor genes of S. populnea were identified. The result showed that four complete length of gustatory receptor genes（ SpopGR1,SpopGR3,SpopGR4 and SpopGR5） were all expressed in antennae and three genes were expressed in leg except SpopGR5. SpopGR1 and SpopGR4 were lowly expressed in female antennae,male antennae,head,chest,abdomen,leg and wing. [Conclusion] Phylogenetic analysis of gustatory receptor genes indicated that SpopGR7,SpopGR9,SpopGR10 may perform the function of bitter taste. This research can promote the understanding of insect gustatory system and establishes a foundation for controlling of S. populnea by chemical ecology measures.

关 键 词：青杨天牛 味觉受体 序列分析 

分 类 号：S763.38[农业科学—森林保护学]