利用GT1-7细胞体外研究雌激素对GnRH分泌及其相关基因表达的影响  

The effect of 17β-estradiol on GnRH secretion and GnRH-related gene expression using GT1-7 cells in vitro

作　　者：陈志龙[1,2] 吴晓敏 郑琛[1] 许晓玲[2] 白佳桦[2] 刘彦[2] 冯涛[2] CHEN Zhi-long;WU Xiao-min;ZHEN Chen;XU Xiao-ling;BAI Jia-hua;LIU Yan;FENG Tao(College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070;Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]北京市农林科学院畜牧兽医研究所,北京100097

出　　处：《生物学杂志》2018年第2期7-11,共5页Journal of Biology

基　　金：国家自然科学基金(31501950);北京市科技新星计划项目(Z141105001814046)

摘　　要：雌激素对GnRH神经元活性及其合成分泌具有重要的调控作用。为了体外模拟雌激素对GnRH合成分泌的抑制作用,使用1μmol/L的雌激素处理GT1-7细胞0、6、12、18、24和30 h,分别检测GnRH的分泌及相关基因GnRH、ERα、KISS-1和GPR54的表达。结果显示:雌激素处理GT1-7细胞GnRH分泌量相对于对照组先升高(6和12 h,P>0.05)后极显著降低(18和30 h,P<0.01)。雌激素处理组GnRH mRNA表达在整个培养期间相对于对照组极显著降低(P<0.01),ERα(12和18 h,P<0.01)、KISS-1(12 h,P<0.01)和GPR54表达也极显著降低(6、12和24 h,P<0.01)。结果表明:1μmol/L雌激素能够抑制GT1-7细胞GnRH的分泌,可能是通过降低ERα、GnRH、KISS-1及GPR54等基因的表达来实现的。Estrogens play essential roles in the neuroendocrine control of reproduction. To simulate inhibiting effects of GnRH secretion and GnRH-related genes（GnRH,ERα,KISS-1 and GPR54） expression by estrogens in vitro,the GnRH-secreting neuronal cell line GT1-7 was exposed to 1 μmol/L estradiol concentrations for 0,6,12,18,24 and 30 h,respectively. The result showed that GnRH secretion of GT1-7 cells treated with 1 μmol/L 17β-estradiol was decreased significantly（18 and 30 h,P〈0. 01）,although slightly increased（6 and 12 h,P〉0. 05） at the beginning of the process. Compared with the control,GnRH mRNA treated with 1 μmol/L estradiol was significantly decreased（P〈0. 01） through all culture period. ERα（12 and 18 h,P〈0. 01）,KISS-1（12 h,P〈0. 01）and GPR54（6,12 and 24 h,P〈0. 01） mRNA were also decreased. It revealed that 1 μmol/L estradiol could inhibit GnRH secretion of GT1-7 cells and this effect may be achieved by down-regulating mRNA of ERα,KISS-1,GnRH and GPR54.

关 键 词：雌激素 GT1-7 GNRH 基因表达 

分 类 号：Q78[生物学—分子生物学]