17种鸡雁类IFNA的分子进化分析  

Molecular evolution of IFNA in seventeen Galloanserae species

作　　者：丁恒武 蒋澜 章勤 王青青 王莹[1] 董锦绣 吴璇 易双龙 陈仁瑞 阚显照[1] DING Heng-wu;JIANG Lan;ZHANG Qing;WANG Qing-qing;WANG Ying;DONG Jin-xiu;WU Xuan;YI Shuang-long;CHEN Ren-rui;KAN Xian-zhao(The Institute of Bioinformatics, College of Life Sciences, Anhui Normal University, Wuhu 241000, China)

机构地区：[1]安徽师范大学生命科学学院生物信息研究所,芜湖241000

出　　处：《生物学杂志》2018年第2期22-28,共7页Journal of Biology

基　　金：安徽高校自然科学研究重大项目(KJ2016SD22);安徽省高等教育振兴计划重大教学改革项目(2015zdjy035);安徽师范大学大学生创新创业训练计划项目(国家级;201510370068);安徽师范大学本科生优秀毕业论文培育计划项目(pyjh2015316)

摘　　要：干扰素是一类具有抗病毒、抗肿瘤、免疫调节等生物学功能的细胞因子。干扰素-α属于Ⅰ型干扰素,由Z染色体连锁基因IFNA编码。利用PCR技术,对雁形目8种鸟类IFNA进行扩增并测序;联合GenBank中释放的数据,利用生物信息学方法对17种鸡雁类IFNA的序列特征、进化速率、编码蛋白的理化性质等进行分析,并使用MP、ML和BI 3种方法构建进化树以探讨其系统发育意义。结果表明大多数鸡雁类IFNA序列中存在CpG island,且该基因编码的干扰素-α为不稳定的亲水性分泌蛋白;与线粒体nad2、cytb和atp6 3种基因相比,鸡雁类IFNA的dN/dS值较高,进化速率较快。此外,在鸡雁小纲类群中发现了IFNA有1个正选择位点。分子系统发育分析表明IFNA带有部分系统发育信息,且多基因联合构建的分子树能较好地反映物种的进化历史。The Interferons（IFN,interferon） are a family of cytokines which have various biological functions,such as anti-virus,antitumor,and immune regulation. Interferon-α belongs to type I interferon,encoded by Z-linked gene IFNA. In this study,the sequences of IFNA in eight avian species of anseriformes were amplified and sequenced by PCR. Combined with the data retrived from Gen Bank,the sequence characterization,the evolutionary rate and the physicochemical properties of IFNA in seventeen species of Galloanserae were analyzed using bioinformatic methods. To discuss the phylogenetic implications of IFNA,we reconstructed the evolutionary trees using maximum parsimony（MP）,maximum likelihood（ML） and bayesian inference（BI） methods. Results demonstrated that the CpG islands were existed in nucleotide sequences of IFNA in majority of Galloanserae species,and detected that the Interferon-α（IFN-α）was unstable hydrophilic secreted proteins. The evolutionary rate of IFNA in Galloanserae was faster than that of three mitochondrial coding genes（nad2,cytb and atp6） with higher dN/dS. In addition,a positive selection site of IFNA was found in Galloanserae species in this study. The molecular phylogenetic analysis indicated that IFNA had some phylogenetic informations within Galloanserae.Phylogenetic tree based on multiple genes could reveal the evolutionary history of species.

关 键 词：IFNA 鸡雁小纲 序列特征 进化速率 系统发育 

分 类 号：Q951.3[生物学—动物学] Q959.7