上海动物园卷羽鹈鹕小种群遗传多样性的初步分析  

Preliminary genetic diversity analysis of the small isolated population of the Dalmatian pelican(Pelecanus crispus) in Shanghai Zoo

作　　者：周颖 马珺[1] 黄康宁[1] 陈晓兰[1] 傅建义 徐正强[1] ZHOU Ying;MA Jun;HUANG Kang-ning;CHEN Xiao-lan;FU Jian-yi;XU Zheng-qiang(Shanghai Zoo, Shanghai 200335, Chin)

机构地区：[1]上海动物园,上海200335

出　　处：《生物学杂志》2018年第2期56-59,共4页Journal of Biology

基　　金：上海市绿化和市容管理局科学技术项目(F090404);上海市绿化和市容管理局科学技术项目(G150407)

摘　　要：通过筛选的12个微卫星基因座位对上海动物园卷羽鹈鹕小种群的遗传多样性进行分析。结果表明:多态信息含量(PIC)=0.60±0.15,等位基因变异程度高,但是基因杂合度只有0.36±0.15,显示该种群遗传变异较小,近亲繁殖效应明显。26个个体相互关系分支图显示,在相似系数为0.77处,树形图分出11个聚类,其中16个个体构成一个大的聚类,全部为卷羽鹈鹕的后代;其余10个聚类的个体后代不同程度上混有白鹈鹕的基因。建议对该种群采取有效的遗传管理,以使其能够健康繁衍。Based on the analysis of 12 microsatellite loci,the genetic diversity of the small isolated population of the Dalmatian pelican（Pelecanus crispus） was studied in Shanghai Zoo. The results showed that the polymorphism information content was 0. 60 ±0. 15,which demonstrated a high degree of allelic variation of the population. The observed heterozygosity was 0. 36 ± 0. 15,which indicated that the population genetic variation had been low,and the inbreeding occurred in the isolated population. The results of clustering analysis showed the 26 individuals could be separated into 11 clusters when the coefficients were 0. 77. One cluster contained of 16 individuals which were pure Dalmatian pelican,and the others were crossed with the Great White pelican（P. onocrotalus） to some extent. It should be taken effective genetic management in order to achieve the sustainable development of isolated pelican population in the Zoo.

关 键 词：卷羽鹈鹕 微卫星 近亲繁殖 种群遗传管理 

分 类 号：Q347[生物学—遗传学]