基于SALF-seq的玉米抗大斑病基因QTL分析  

QTL Analysis of North Corn Leaf Blight by SALF-seq

作　　者：马骏[1] 刘欣芳[1] 齐欣[1] 弓雪[1] 李明[1] 姜敏[1] MA Jun;LIU Xin-fang;QI Xin;GONG Xue;LIMing;JIANG Min(Maize Research Institute of Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning 110161)

机构地区：[1]辽宁省农业科学院玉米研究所,辽宁沈阳110161

出　　处：《辽宁农业科学》2018年第2期8-13,共6页Liaoning Agricultural Sciences

基　　金：农业部转基因生物新品种培育重大专项(2016ZX08003004-007);辽宁省科技厅项目(2014201003)

摘　　要：利用SLAF-seq(Specific-Locus Amplified Fragment Sequencing)技术对玉米大斑病抗、感亲本A619 Ht2/辽3162的BC1遗传分离群体(2个亲本和50+50的混池)进行关联分析,SNP_index和ED(Euclidean distance)两种关联分析法共定位到2号染色体上,从201819690到205452750,大小为3.63Mb,其间定位到的基因有110个。该研究成果为进一步进行玉米大斑病抗病基因研究提供了理论和数据支撑。The susceptible inbred line Liao 3162,high resistance inbred line A619 Ht2 and mixing pool of parents（ 50 susceptible and 50 resistant populations of BC1 F1） were carried outassociation analysis by using simplified genome technology. The common region obtained by SNP and Euclidean distance methods is 3. 63 Mb（ 201819690 to 205452750 bp） and contains 110 genes in chr 2. The results provide theoretical and data supports for furtherstudying maize resistance gene.

关 键 词：玉米大斑病 抗性基因 SNP 

分 类 号：Q343.17[生物学—遗传学]