利用电化学法结合丝素膜调控羟基磷灰石沉积及其形貌  

Electrochemical deposition technology regulating the morphology of hydroxyapatite deposited on silk fibroin film

作　　者：王天云 张青[1] 朱良均[1] 杨明英[1] WANG Tianyun;ZHANG Qing;ZHU Liangjun;YANG Mingying(Institute of Applied Bioresource Research, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China)

机构地区：[1]浙江大学动物科学学院应用生物资源研究所,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2018年第2期209-214,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-18);浙江省自然科学基金重点项目(LZ17C170002);中央高校基本科研业务费专项资金(2018XZZX001-111)

摘　　要：为研究电化学沉积法结合天然丝素蛋白(silk fibroin,SF)调控羟基磷灰石(hydroxyapatite,HAp)在丝素表面的沉积形貌,探讨沉积时间、电流密度和电解液p H对沉积HAp形貌的影响,通过扫描电子显微镜(scanning electron microscope,SEM)、电子衍射能谱(electron diffraction spectroscopy,EDS)、傅里叶变换红外光谱分析(Fourier transform infrared spectroscopy,FTIR)和X射线衍射来表征沉积HAp的形貌、Ca/P、化学结构和晶相结构。结果表明:沉积层随沉积时间的延长而增厚,且沉积颗粒和孔径随电流密度和电解液p H值的增大而增大;当电流密度为2.5 m A/cm2、电解液p H为5.3、沉积时间为30 min时,能得到均一平整的纳米多孔HAp涂层。本研究拓展了HAp/SF复合材料在骨组织工程领域的研究和应用,为开发丝素蛋白骨组织修复材料提供了新思路。In order to investigate the morphology of hydroxyapatite（HAp） on the surface of silk fibroin（SF） film regulated by electrochemical deposition process, the effects of deposition time, ampere density and electrolyte p H on HAp morphology were discussed. Scanning electron microscope（SEM）, electron diffraction spectroscopy（EDS）,Fourier transform infrared spectroscopy（FTIR） and X-ray diffractions（XRD） were applied to characterize the morphology, Ca/P, chemical structure and crystalline phase of HAp. The results indicated that the thickness of HAp was increased by prolongation of deposition time, and the size of synthesized particles and pores was also increased with the rise of ampere density and electrolyte p H. A uniform, flat and porous surface of HAp was obtained with the ampere density of 2.5 m A/cm2, electrolyte p H of 5.3, deposition time of 30 min. This study expands the research and application of HAp/SF biomaterials in bone tissue engineering, and provides novel ideas for developing bone repairing materials based on silk fibroin.

关 键 词：羟基磷灰石 丝素蛋白 电化学沉积 骨修复 

分 类 号：R318.08[医药卫生—生物医学工程]