组织芯片结合图像分析技术研究兔死后DNA不同时间段的变化  

Study on the Changes of DNA after Death in Rabbits by Tissue Microarray and Image Analysis Technology

作　　者：罗国厂[1] 杨坦[1] 张春玉[1] 吴新杰 熊平[3] Luo Guochang;et al(NanYang Medical College, Nanyang 47300)

机构地区：[1]南阳医学高等专科学校,南阳473000 [2]内乡县人民法院技术科 [3]南华大学医学院

出　　处：《数理医药学杂志》2018年第5期633-635,共3页Journal of Mathematical Medicine

基　　金：南阳市科技攻关计划项目(编号:2011GG031)

摘　　要：目的:测量组织中DNA含量变化,研究不同时间段、不同组织DNA含量变化,与已知人体组织相比较,检验其用于推断死亡经过时间的可能。方法:利用高通量的组织芯片技术,对Feulgen染色后组织芯片细胞DNA用细胞图像分析技术定量分析。结果:兔子死亡后各组织的DNA发生降解,一定时间内DNA降解出现平台期,各组织DNA降解改变平均值与死后经过时间之间都存在显著相关性(P<0.05),气管、延髓、软骨组织表现更明显,与人体组织相比较,确定兔组织DNA作为参照的可行性。Objective：To measure the changes of DNA content in tissues,to study the changes of DNA content in different tissues in different time periods,and to test the possibility of using them to infer the elapsed time of death compared with known human tissues.Methods：Using high-throughput tissue microarray technique,the quantitative analysis of cell image analysis for DNA of tissue chip cells stained with Feulgen was carried out.Results：The DNA content of rabbit tissues after death was degraded,there was a significant correlation between the mean value of DNA degradation and postmortem elapsed time（P〈0.05）.The trachea,medulla oblongata and cartilage were more obvious.Compared with human tissues,the feasibility of DNA in rabbit tissues was determined.

关 键 词：法医病理学 死亡时间 DNA 组织芯片技术 图像分析技术 

分 类 号：D919[医药卫生—法医学]