生长激素调控PCOS大鼠血清与卵巢组织中血管因子表达的研究  

Effects of growth hormone on the expression of vascular factors in serum and ovarian tissue of PCOS rats

作　　者：王施元 李肖肖 于彩虹 王珊 尹晓静 刘羽[6] 王芳芳[6] 王瑞静 刘梅梅[6] WANG Shi yuan;LI Xiao- xiao;YU Cai-hong;WANG Shan;YIN Xiao-jing;LIU Yu;WANG Fang-fang;WANG Rui-jing;LIU Mei-mei(Harbin Medical University ,Harbin 150000;Department of Obstetrics ＆Gynecology ,Affiliated Hospital of Jining Medical College ,Jining 272029;Department of Obstetrics ＆ Gynecology ,the Fourth Hospital of Shijiazhuang, Shijiazhuang 050000;Department of Obstetrics＆ Gynecology, the First People ＇s Hospital of Xinxiang City, Xinxiang 453000 5. Department of Obstetrics＆ Gynecology, Central Hospital of Chaoyang City,Chaoyang 122000 6. Department of Obstetrics ＆ Gynecology, the Second Affiliated Hospital of Harbin Medical University, Harbin 150000)

机构地区：[1]哈尔滨医科大学,哈尔滨150000 [2]济宁医学院附属医院妇产科,济宁272029 [3]石家庄市第四医院妇产科,石家庄050000 [4]新乡市中心医院妇产科,新乡453000 [5]朝阳市中心医院妇产科,朝阳122000 [6]哈尔滨医科大学附属第二医院,哈尔滨150000

出　　处：《生殖医学杂志》2018年第5期444-450,共7页Journal of Reproductive Medicine

摘　　要：目的探讨来曲唑诱导的多囊卵巢综合征(PCOS)大鼠血清与卵巢组织中内皮抑素(ES)、内皮素-1(ET-1)和血小板反应素-1(TSP-1)的表达及意义,并对比给予生长激素前后ES、ET-1和TSP-1的表达变化。方法选取6周龄清洁雌性SD大鼠70只,分为对照组20只,模型组50只。模型组给予来曲唑造模,剂量1mg/(kg·d),连续灌胃21d建立PCOS大鼠模型,对照组给予等剂量1%羧甲基纤维素灌胃。随机选取模型组10只大鼠评估造模成功后,模型组余下大鼠随机分为生长激素组(GH组,20只)和PCOS组(20只)。GH组皮下注射重组人生长激素(rhGH),剂量为1U/(kg·d),PCOS组和对照组皮下注射与rhGH等量的生理盐水,均21d后处死,留取各组大鼠血清样本及卵巢组织。采用ELISA法、免疫组织化学法检测各组大鼠血清、卵巢组织中ES、ET-1和TSP-1的表达。结果与对照组相比,PCOS组大鼠血清及卵巢组织中ES和ET-1均表达增加(P<0.05),TSP-1表达降低(P<0.05);与PCOS组大鼠相比,GH组大鼠血清及卵巢组织中ES和ET-1表达降低(P<0.05),而TSP-1表达增加(P<0.05)。结论 ES、ET-1及TSP-1在PCOS的发生发展中发挥着重要作用,GH可能通过调节血清及卵巢组织中ES和ET-1的表达而在PCOS中发挥作用。Objective：To explore the expression and significance of endostatin（ES）,endothelin-1（ET-1）and thrombocytopenin-1（TSP-1）in the serum and ovary tissues of rats with polycystic ovary syndrome（PCOS）induced by letrozole,and compare the changes of ES,ET-1 and TSP-1 before and after administration of growth hormone.Methods：Seventy female SD rats of six weeks age were randomly divided into control group（n=20）and model group（n=50）.In model group,the rate were administered letrozole by intragastric infusion with a dose of 1 mg/（kg·d）for 21 days to create PCOS model.The control group was given equal dose of 1% carboxymethyl cellulose.Ten rats were randomly selected to evaluate the success of establishment of model in model group,and the rest of rats were randomly divided into growth hormone group（GH group,n=20）and PCOS group（n=20）.The GH group was injected subcutaneously with recombinant human growth hormone（rhGH）at a dose of 1 U/（kg.d）,and the rats in PCOS group and the control group were injected with equal dose of normal saline.All the rats were killed after 21 days,the serum samples and ovarian tissue were collected.The expression of ES,ET-1 and TSP-1 in serum and ovarian tissues of rats were detected by ELISA and immunohistochemistry.Results：Compared with control group,the expressions of ES and ET-1 were significantly higher in serum and ovary tissues of PCOS rats（P〈0.05）and expression of TSP-1 was significantly lower（P〈0.05）.Compared with group PCOS rats,the expressions of ES and ET-1 in serum and ovary tissue of GH group were significantly decreased（P〈0.05）,while the expression of TSP-1 was significantly increased（P〈0.05）.Conclusions：ES,ET-1 and TSP-1 play important roles in the development of PCOS.GH may play a role in PCOS by regulating the expression of ES and ET-1 in serum and ovarian tissue.

关 键 词：多囊卵巢综合征 内皮抑素 内皮素-1 血小板反应素-1 生长激素 

分 类 号：R711.75[医药卫生—妇产科学]