男性不育症患者GST基因多态性与精子质量、氧化应激、细胞凋亡的相关性研究  

The correlation of GST gene polymorphism with sperm quality,oxidative stress and apoptosis in patients with male infertility

作　　者：梁嘉颖[1] 郑毅春[1] 李子涛[1] 张杰[1] 肖宗辉[1] 汪李虎[1] LIANG Jia-ying;ZHENG Yi-chun;LI Zi-tao;ZHANG Jie;XIAO Zong-hui;WANG Li-hu(Reproductive Health and Infertility Department, Guangdong Maternal and Child Health Hospital, Guangdong, Guangzhou 510010, China)

机构地区：[1]广东省妇幼保健院生殖健康与不孕症科,广东广州510010

出　　处：《海南医学院学报》2018年第6期719-722,726,共5页Journal of Hainan Medical University

基　　金：广州市科技计划项目(201707010375)~~

摘　　要：目的:研究男性不育症患者谷胱甘肽-S-转移酶(GST)基因多态性与精子质量、氧化应激、细胞凋亡的相关性。方法:纳入本院首次诊断为男性不育症伴少弱精子症的患者作为不育组并根据GST基因GSTM1及GSTT1亚型缺失的多态性分为GSTM1/T1[+/+]、GSTM1/T1[+/-]、GSTM1/T1[-/+]、GSTM1/T1[-/-],测定精液质量的参数以及精液中氧化应激指标、细胞凋亡指标的含量。结果:GSTM1/T1[+/-]、GSTM1/T1[-/+]、GSTM1/T1[-/-]患者的精子浓度、精子活力以及精液中Nme5、PKC、ERK、TA、Bcl-2的含量均显著低于GSTM1/T1[+/+]患者,精液中MDA、AOPP、AIF、Caspase-9、Caspase-3的含量高于GSTM1/T1[+/+]患者(P<0.05);GSTM1/T1[-/-]患者的精子浓度、精子活力以及精液中Nme5、PKC、ERK、TA、Bcl-2的含量均显著低于GSTM1/T1[+/-]、GSTM1/T1[-/+]患者,精液中MDA、AOPP、AIF、Caspase-9、Caspase-3的含量高于GSTM1/T1[+/-]、GSTM1/T1[-/+]患者(P<0.05)。结论:男性不育症患者GST基因GSTM1及GSTT1亚型的缺失会造成精子质量低下并激活氧化应激及细胞凋亡。Objective：To study the correlation of glutathione S-transferase（GST）gene polymorphism with sperm quality,oxidative stress and apoptosis in patients with male infertility.Methods：Patients who were diagnosed with male infertility combined with oligoasthenozoospermia for the first time in Guangdong women and children hospital between July 2014 and June2017 were enrolled in the infertility group and divided into GSTM1/T1[＋/＋],GSTM1/T1[＋/-],GSTM1/T1[-/＋]and GSTM1/T1[-/-]according to the polymorphism of GST gene GSTM1 and GSTT1 subtype deletion,and the semen quality parameters as well as the contents of oxidative stress indexes and apoptosis indexes in semen were determined.Results：The sperm concentration and sperm motility as well as the Nme5,PKC,ERK,TA and Bcl-2 levels in semen of patients with GSTM1/T1[＋/-],GSTM1/T1 [-/＋]and GSTM1/T1 [-/-]were significantly lower than those of patients with GSTM1/T1[＋/＋]whereas MDA,AOPP,AIF,Caspase-9 and Caspase-3 levels in semen were higher than those of patients with GSTM1/T1[＋/＋]（P〈0.05）;the sperm concentration and sperm motility as well as the Nme5,PKC,ERK,TA and Bcl-2 levels in semen of patients with GSTM1/T1[-/-]were significantly lower than those of patients with GSTM1/T1[＋/-]and GSTM1/T1 [-/＋]whereas MDA,AOPP,AIF,Caspase-9 and Caspase-3 levels in semen were higher than those of patients with GSTM1/T1 [＋/-]and GSTM1/T1 [-/＋]（P〈0.05）.Conclusion：The deletion of GST gene GSTM1 and GSTT1 subtypes in patients with male infertility can lead to low sperm quality and activate the oxidative stress and apoptosis.

关 键 词：男性不育症 谷胱甘肽-S-转移酶 多态性 氧化应激 细胞凋亡 

分 类 号：R698.2[医药卫生—泌尿科学]