糖尿病合并牙周炎患者龈沟液中OPG/RANKL表达与炎症因子、自由基生成及骨代谢的相关性  

The correlation of OPG/RANKL expression in gingival crevicular fluid with inflammatory factors,free radical generation and bone metabolism in patients with diabetes mellitus complicated by periodontitis

作　　者：郝瑞 贺菲菲 梁艳 魏媛媛 高婷婷 HAO Rui;HE Fei-fei;LIANG Yan;WEI Yuan-yuan;GAO Ting-ting(Stomatology Department, Xi＇an No.4 Hospital, Xi＇an 710100, Shaanxi Province, China;Endocrine Department, Xi＇an No.4 Hospital, Xi＇an 710100, Shaanxi Province, China;Department of Oral Medicine, Cangzhou Dental Hospital, Cangzhou 061000, Hebei Province, China;Department of Internal Medicine, Xi＇an Weapons Industry 521 Hospital, Xi＇an 7100G5, Shaanxi Province, China;Stomatology Department, Ximei Hospital, Xi＇an 7100G5, Shaanxi Province, China)

机构地区：[1]西安市第四医院口腔科,陕西西安710004 [2]西安市第四医院内分泌科,陕西西安710004 [3]沧州市口腔医院口腔内科,河北沧州061000 [4]西安兵器工业五二一医院内科,陕西西安710065 [5]西美口腔科,陕西西安710065

出　　处：《海南医学院学报》2018年第5期585-588,共4页Journal of Hainan Medical University

基　　金：西安市卫生局科研项目(J201602037)~~

摘　　要：目的:研究糖尿病合并牙周炎患者龈沟液中OPG/RANKL表达与炎症因子、自由基生成及骨代谢的相关性。方法:选择在西安市第四医院就诊的糖尿病合并牙周炎患者作为研究的牙周炎组,选择同期接受牙周检查的健康志愿者作为对照组。采集龈沟液并测定OPG/RANKL的表达量、炎症因子及骨代谢分子的含量、自由基的生成量。结果:牙周炎组龈沟液中OPG的蛋白表达量及OPG/RANKL表达量的比值、ICTP、Runx2的含量均显著低于对照组,RANKL的蛋白表达量、TNF-α、IL-1β、IL-17、HMGB1、DKK1、MMP1的含量及ROS、MDA、8-OHdG的生成量均显著高于对照组;牙周炎组患者龈沟液中OPG/RANKL表达量的比值与TNF-α、IL-1β、IL-17、HMGB1、DKK1、MMP1的含量及ROS、MDA、8-OHdG的生成量呈负相关关系,与ICTP、Runx2的含量呈正相关关系。结论:糖尿病合并牙周炎患者牙周组织中炎症及氧化应激反应的过度激活能够引起OPG/RANKL表达紊乱,进而造成骨代谢异常。Objective：To study the correlation of OPG/RANKL expression in gingival crevicular fluid with inflammatory factors,free radical generation and bone metabolism in patients with diabetes mellitus complicated by periodontitis.Methods：Patients with diabetes mellitus complicated by periodontitis who were treated in Xi’an fourth hospital between March 2015 and May 2017 were selected as the periodontitis group for the research,and healthy volunteers who underwent periodontal examination during the same period were selected as the control group.The gingival crevicular fluid was collected to determine the expression of OPG/RANKL,the contents of inflammatory factors and bone metabolism molecules as well as the generation of free radicals.Results：OPG protein expression,OPG/RANKL expression ratio as well as ICTP and Runx2 contents in gingival crevicular fluid of periodontitis group were significantly lower than those of control group whereas RANKL protein expression,TNF-α,IL-1β,IL-17,HMGB1,DKK1 and MMP1 contents as well as ROS,MDA and 8-OHdG generation were significantly higher than those of control group;OPG/RANKL expression ratio in gingival crevicular fluid of periodontitis group was negatively correlated with TNF-α,IL-1β,IL-17,HMGB1,DKK1 and MMP1 contents as well as ROS,MDA and 8-OHdG generation,and positively correlated with ICTP and Runx2 contents.Conclusion：The excessive activation of inflammation and oxidative stress in periodontal tissue of patients with diabetes mellitus complicated by periodontitis can cause OPG/RANKL expression disorder and then lead to bone metabolism disorder.

关 键 词：牙周炎 骨保护素 核因子ΚB受体活化因子 炎症反应 自由基 骨代谢 

分 类 号：R587.1[医药卫生—内分泌] R781.42[医药卫生—内科学]