新疆南疆牛冠状病毒BCV-Aks-01株分离及基因型鉴定  

Isolation and Genotype Identification of Bovine Coronavirus BCV-Aks-01 Strain in Southern Xinjiang

作　　者：张莹钰[1] 张迎春[2] 王青青[3] 胡建军[3] 王娜[3] 彭安业[2] 张婉琪[1] 崔鑫[3] ZHANG Ying-yu;ZHANG Ying-chun;WANG Qing-qing;HU Jian-jun;WANG Na;PENG An-ye;ZHANG Wan-qi;CUI Xin(College of life Science, Tarim University, Alar 843300, China;The Veterinary Station of the First Division, Xinjiang Production and Construction Group, Alar 843300, China;College of Animal Science ,Tarim University, Alar 843300, China)

机构地区：[1]塔里木大学生命科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团第一师畜牧兽医工作站,新疆阿拉尔843300 [3]塔里木大学动物科学学院,新疆阿拉尔843300

出　　处：《中国兽医杂志》2018年第2期12-14,18,F0002,共5页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(31660725);新疆生产建设兵团第一师科技局资助项目(2016XS03);新疆生产建设兵团科技局现代农业科技攻关与成果转化资助项目(2016AC012)

摘　　要：将疑似感染牛冠状病毒(Bovine coronavirus,BCoV)病死犊牛的粪便样品,滤菌处理后接种于HCT-8细胞,盲传数代,出现细胞病变后收获细胞液。提取病毒RNA,依据NCBI登录的牛冠状病毒高度保守N基因设计1对特异性引物,对N基因进行RT-PCR扩增、测序及序列分析。结果显示,BCV-Aks-01毒株与牛冠状病毒参考株N基因核苷酸同源性为97.5%~98.6%,同BCoV参考毒株Mebus同源性为97.8%,N基因遗传进化树表明,BCV-Aks-01毒株与BCoV参考株亲缘关系较近,处于遗传进化树中同一分支上,属于冠状病毒Betacoronavirus,证实该分离株为牛冠状病毒。The filtrating fecal Suspension from a suspected infected Bovine coronavirus calf was inoculated into HCT-8 cells. Af- ter blind passages, virus solution was harvested when the CPE appeared. RNA sample was extracted and detected by RT-PCR with special primers. The amplified fragments were sequenced. Analysis of the nucleotide sequences and construction of phylogenetic tree were based on N gene of coronavirus with computer programs. The sequencing results showed that the N gene sequence of BCV-Aks- 01 strain had 95% similarity to the reference strain from GenBank.. The genotyping of BCV-Aks-01 strain belonged to Betacoronavirus of Bovine coronavirus. The result showed that BCV-Aks-01 strain belonged to Bovine coronavirus.

关 键 词：牛冠状病毒 病毒分离 鉴定 N基因 

分 类 号：S855.3[农业科学—临床兽医学]