小反刍兽疫病毒对鼠源树突状细胞成熟分化的影响  

Effects of peste des petits ruminants virus on the maturation and differentiation of murine bone marrow derived dendritic cells

作　　者：李玲霞 吴锦艳[1] 杜国玉 冯倩 王光祥[1] 陈研[1] 曹小安[1] 刘永生[1] 刘湘涛[1] 尚佑军[1] LI Lingxia;WU Jinyan;DU Guoyu;FENG Qian;WANG Guangxiang;CHEN Yan;CAO Xiao＇ an;LIU Yongsheng;SLIU Xiangtao;HANG Youjun(State Key Laboratory of Pathogen Biology of Veterinary Diseases ＆ Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences ＆ Key Laboratory of Animal Virology, Ministry of Agriculture, Lanzhou 730046, Chin)

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《免疫学杂志》2018年第5期369-377,共9页Immunological Journal

基　　金：国家重点研发计划课题(2017YFD0500903);国家现代肉羊产业技术体系项目(CARS-39-04B)

摘　　要：目的研究和探讨小反刍兽疫病毒(PPRV)对小鼠骨髓来源的树突状细胞(BM-DCs)成熟和分化功能的影响。方法从小鼠骨髓中分离得到DCs,经过IL-4和GM-CSF诱导成熟;流式细胞术检测DCs表面共刺激分子表达及吞噬FITC-Dextran的能力;ELISA检测细胞因子IL-6、IL-12p40、IL-1β和IL-10的表达水平。结果利用LPS和PPRV处理DCs 24 h,发现与阳性对照LPS组相比,PPRV作用后显著抑制了CD40和MHC-Ⅱ的表达(P<0.05);滴度适度的PPRV可以显著增加CD40、CD80和CD86的表达,显著升高IL-6和IL-10的分泌(P<0.05),吞噬FITC-Dextran的能力并无显著差异,各组之间也无影响,且不同比例的PPRV对DC存活率无显著影响(P>0.05)。结论 PPRV在一定滴度范围内具有潜在的促进小鼠DC成熟的功能,为免疫学研究奠定基础。This study aims to investigate the effect of pests des petits ruminants virus（PPRV） on thematuration and differentiation of dendritic cells（DCs） derived from murine bone marrow（BM-DC）. DCs wereinduced from Balb/c mice bone marrow in the presence of rm IL-4 and rm GM-CSF, and then subjected to flowcytometry for the expression of costimulatory molecules and the ability of FITC-Dextran phagocytosis. ELISA wasapplied to detect the production of cytokines IL-6, IL-12 p40, IL-1β and IL-10. Data showed that PPRV inhibitedthe expression of CD40 and MHC-II（P0.05）, but increased the levels of IL-6 and IL-10 of DM-DCs, ascompared LPS-treated BM-DCs. Interestedly, diluted PPRV（10-1, 10-2, 10-3） promoted the expression of CD40,CD80 and CD86, as well as the secretion of IL-6 and IL-10（P0.05）, but exerted no effect on FITC-Dextranphagocytosis of BM-DCs, as compared LPS-treated BM-DCs. And the diluted PPRV at different proportion showedon significant difference in the survival rate of DC（P0.05）. From the experiment we draw the conclusion that PPRVin a certain range titer maybe has potential function to promote the maturation of BM-DC, which would lay afoundation for further immunological research.

关 键 词：小反刍兽疫病毒 BM-DC 细胞因子 流式细胞术 

分 类 号：S811.6[农业科学—畜牧学]