两种交联技术处理的兔去细胞颈动脉性能比较  

Properties of decellularized rabbit carotid artery with two different cross-linked technologies:A comparative study

作　　者：黄磊[1] 匡锋[2] 孔德淼[1] 周新民[1] HUANG Lei;KUANG Feng;KONG Demiao;ZHOU Xinmin(Department of Cardiovascular Surgery, Second Xiangya Hospital, Central South University, Changsha 410011;Department of Cardiac Surgery, First Affiliated Hospital of Xiamen University, Xiamen Fujian 361001, China)

机构地区：[1]中南大学湘雅二医院心血管外科,长沙410011 [2]厦门大学附属第一医院心脏外科,福建厦门361001

出　　处：《中南大学学报（医学版）》2018年第4期441-446,共6页Journal of Central South University ：Medical Science

基　　金：厦门市科技计划项目(3502Z20154001)~~

摘　　要：目的:比较兔去细胞颈动脉采用不同交联技术处理后的血管性能差别。方法:将兔去细胞颈动脉随机分成光氧化组和原花青素组,分别采用染料介导的光氧化交联技术、原花青素交联技术处理。对光氧化组和原花青素组分别进行热皱缩温度、最大抗张强度及最大拉伸距离的血管体外性能检测,以及血管形态、炎症反应及钙化情况的血管体内性能检测。结果:光氧化组与原花青素组兔去细胞颈动脉,在血管体外性能方面,热皱缩温度、最大抗张强度和最大拉伸距离差异无统计学意义(P>0.05)。体内性能方面,两组在血管组织结构、炎症反应方面相似,VonKossa钙染色均未见明显钙化斑块,但原花青素组的钙含量明显低于光氧化组(P<0.05)。结论:两种交联技术处理的兔去细胞颈动脉均保持了与新鲜血管相似的物理性能,具有较好的抗钙化能力和较低的免疫原性;原花青素交联技术处理的血管抗钙化能力更佳。Objective： To compare the properties between decellularized rabbit carotid artery with diff erent cross-linked technologies.Methods： The decellularized rabbit carotid arteries were randomly divided into a photo-oxidation group and a procyanidins group. One group was cross-linked with photo-oxidation and the other group was cross-linked with procyanidins. The in vitro or in vivo properties of the two groups were evaluated by testing heat-shrinking temperature, max tensile strength and the max elongation or by testing tissue structure, inf lammatory reaction and calcification degree.Results： The heat-shrinking temperature, max tensile strength and the max elongation were similarin the two groups（P0.05）. The tissue structure and inflammatory reaction were also similar in the two groups. Although the result of Von-Kossa calcium salt stain was slightly different, the calcium content was lower in the procyanidins group than that in the photo-oxidation group（P0.05）.Conclusion： The grafts by two cross-linked technologies show excellent mechanical capability, lower immunogenicity, good biological stability and anti-calcification ability. The procyanidins group shows a better anti-calcification property than the photo-oxidation group.

关 键 词：去细胞 光氧化 原花青素 抗钙化 交联技术 

分 类 号：R54[医药卫生—心血管疾病]