养殖大菱鲆感染迟缓爱德华氏菌的分离、毒力基因及ERIC-PCR分析  

Isolation,virulence analysis and ERIC-PCR genotyping of Edwardsiella tarda isolates from farmed Turbot Scophthalmus maximus(L.)

作　　者：于新然 姚洪[1] 叶仕根[1] 黎睿君[1] 李华[1] 李强[1,2] YU Xin-ran;YAO Hong;YE Shi-gen;Ll Rui-jun;Ll Hua;Ll Qiang(Key Laboratory of Mariculture ＆ Stock Enhancement in North China’s Sea,Ministry of Agriculture,Dalian Ocean University,Dalian 116023, Ghina;College of Marine and Biology Engineering,Yancheng lnstitute of Technology,Yancheng 224051,China)

机构地区：[1]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 [2]盐城工学院海洋与生物工程学院,江苏盐城224051

出　　处：《大连海洋大学学报》2018年第2期169-174,共6页Journal of Dalian Ocean University

基　　金：国家海洋公益性行业科研专项(201405003);辽宁省海洋渔业厅重点攻关项目(201402)

摘　　要：为了调查辽宁省葫芦岛市养殖患病大菱鲆Scophthalmus maximus病原菌感染情况,选用迟缓爱德华氏菌Edwardsiella tarda特异性引物对分离菌株进行PCR鉴定,共分离得到22株迟缓爱德华氏菌。结果表明:迟缓爱德华氏菌毒力基因的PCR扩增显示,22株菌均含有kat B、fim A、gad B、esa V等基因;人工感染试验表明,22株迟缓爱德华氏菌感染并致大菱鲆累积死亡率均为100%;ERIC-PCR结果显示,迟缓爱德华氏菌模式菌株(ATCC15947)与分离株可分为2个基因型,分别标记为Ⅰ和Ⅱ型,其中22株迟缓爱德华氏菌分离株均为Ⅱ型,与ATCC15947菌株为Ⅰ型明显不同。本研究结果为养殖大菱鲆迟缓爱德华氏菌感染的防控提供了依据。Pathogen Edwardsiella tarda was isolated from diseased farmed turbot Scophthalmus maximus（ L.）,identified by PCR with a specific primer and further genotyping by ERIC-PCR analysis to explore the pathogenic bacteria infection of diseased farmed turbot. The PCR amplification of virulence gene of the pathogen E. tarda revealed that all the 22 isolates contained the four tested virulence genes（ kat B,fim A,gad B and esa V）. Challenge tests showed that the cumulative mortality of 100% was observed in the turbot challenged with the 22 strains of pathogen. In addition,ERIC-PCR showed that the standard E.tarda（ ATCC15947） and all 22 isolates were divided into two genotypes including E.tarda Ⅰ and E.tarda Ⅱ. The reference strains belonged to E.tarda Ⅰ,and the isolates belonged to E.tarda Ⅱ. The findings will provide very important references for disease prevention in turbot.

关 键 词：大菱鲆 迟缓爱德华氏菌 毒力分析 ERIC-PCR 

分 类 号：S943[农业科学—水产养殖]