调脂积冲剂对痰瘀互结证代谢综合征患者肠道菌群结构的影响  

Effect of Tiaozhiji Granules on intestinal flora structure in patients with metabolic syndrome and syndrome of intermingled phlegm and blood stasis

作　　者：罗科学[1] 潘智敏[2] 张永乐[1] 李宏春[1] 贾晓雁[1] LUO Ke-xue;PAN Zhi-min;ZHANG Yong-le(Depariment of Geriatrics, Tongde Hospital of Zhejiaag Province, Hangzhou, Zhejiang 310032, China)

机构地区：[1]浙江省立同德医院老年医学科,浙江杭州310032 [2]浙江省中医院干部科,浙江杭州310006

出　　处：《中华全科医学》2018年第5期744-747,共4页Chinese Journal of General Practice

基　　金：浙江省中医药管理局科研基金项目(2016ZA004)

摘　　要：目的研究调脂积冲剂对痰瘀互结证代谢综合征(metabolic syndrome,MS)患者肠道菌群结构的影响。方法选取2016年4月1日—2017年3月30日浙江省立同德医院收治的痰瘀互结证MS患者57例,按照治疗方法不同分为调脂积组27例和西药组30例,调脂积组采用调脂积冲剂治疗,西药组采用西药二甲双胍片治疗,同时选择健康志愿者30例为空白对照组。荧光定量PCR法定量分析3组人群肠道主要菌群结构的变化。结果MS组患者乳酸杆菌、双歧杆菌、丁酸盐产生菌含量均明显低于对照组(P<0.05),肠杆菌、硫酸盐还原菌含量明显高于对照组(P<0.05),肠道总菌含量2组间比较差异无统计学意义(P>0.05);2组患者治疗后乳酸杆菌、双歧杆菌含量较治疗前均明显升高(P<0.05),肠杆菌、硫酸盐还原菌较治疗前明显下降(P<0.05),丁酸盐产生菌、肠道总菌较治疗前差异无统计学意义(P>0.05),调脂积组患者乳酸杆菌含量明显高于西药组(P<0.05),肠杆菌含量明显低于西药组(P<0.05),双歧杆菌、丁酸盐产生菌、硫酸盐还原菌、肠道总菌含量较西药组差异无统计学意义(P>0.05)。结论 MS患者的肠道菌群的结构发生了改变,调脂积冲剂能通过促进乳酸杆菌、双歧杆菌的生长,抑制硫酸盐还原菌和肠杆菌的生长,改善肠道菌群结构,发挥其治疗MS的作用,值得在临床上推广应用。Objective To study the effect of Tiaozhi Recipes on intestinal microflora of patients with metabolic syndrome （MS） with phlegm and blood stagnation. Methods Fifty-seven patients with metabolic syndrome with phlegm, blood stasis and cross-over syndrome treated in our hospital from April, 2016 to March, 2017 were divided into two groups ac- cording to different treatment methods： Tiaozhiji group （27 cases） and western medicine group （30 cases）. The Tiaozhiji group was treated with Tiaozhi capsule, Western medicine group treated with metformin tablets, while 30 healthy volun- teers were selected as blank control group. Fluorescent quantitative PCR method was used to quantitatively analyze the changes of intestinal flora in three groups. Results The Lactobacillus, Bifidobacterium, butyrate-producing bacteria in MS group were significantly lower than those in the control group （ P 〈 0.05 ）. Enterobacter, sulfate-reducing bacteria was significantly higher than the control group （ P 〈 0.05 ）, the total intestinal bacterial content between the two groups was no significant difference （ P 〉 0. 05 ） ; Laetobaciflus, Bifidobacterium content in both groups after treatment were sig- nificantly higher than those before treatment （ P 〈 0. 05 ） , Enterobacteriaceae, sulfate-reducing bacteria were significantly decreased than those before treatment （ P 〈 0.05 ） , there were no significantly difference about butyrate-producing bacte- ria, intestinal bacteria than before treatment （ P 〉 0. 05 ） , the content of Lactobacillus in the Tiaozhiji group was signifi- cantly higher than that in the western medicine group （ P 〈 0. 05 ）, and the content of Enterobacter in the Tiaozhiji group was significantly lower than that of western medicine group （ P 〈 0. 05 ）, Bifidobacterium, butyrate-producing bacteria, sulfate-reducing bacteria and intestinal bacteria was not statistically difference significant between the two groups （P 〉 0. 05 ）. Conclusion The structure of intesti

关 键 词：代谢综合征 肠道菌群 痰瘀互结证 调脂积冲剂 荧光定量PCR 

分 类 号：R589[医药卫生—内分泌] R242[医药卫生—内科学]