ω-3鱼油脂肪乳注射液对中暑大鼠动脉血相关指标的影响及其机制  

Effect and mechanism of ω-3 fatty acids on factors in arterial blood in heat stroke rats

作　　者：胡良英[1] 寿飞燕[1] 蒋景华[1] 孙红华 李玉红[3] HU Liang-ying;SHOU Fei-yan;JIANG Jing-hua(Department of Convenient Outpatient Service, Shaoxing People＇s Hospital, Shaoxing, Zhejiang 312000, China)

机构地区：[1]绍兴市人民医院方便门诊,浙江绍兴312000 [2]温州医科大学附属第二医院育英儿童医院放射科,浙江温州325000 [3]绍兴市人民医院麻醉科

出　　处：《中华全科医学》2018年第5期752-753,808,共3页Chinese Journal of General Practice

基　　金：2016年浙江省医药卫生一般研究计划(2016-KYB-297)

摘　　要：目的探讨ω-3鱼油脂肪乳注射液对动脉血相关指标的影响及其机制。方法将90只SD大鼠按随机数字表法分为3个组,分别为正常对照组、中暑模型组、ω-3鱼油脂肪乳注射液治疗组,每组30只大鼠。将大鼠置于40℃,60%相对湿度的恒温箱中1 h复制中暑模型。ω-3鱼油脂肪乳治疗组于制模后尾部静脉注射ω-3鱼油脂肪乳注射液1.67 ml/(kg·d);正常对照组和中暑模型组尾部静脉均注射同等剂量的生理盐水,2组均每日1次,共3 d。各组于制模后12、24、48 h记录大鼠的体温、心率、平均动脉压(MAP);在以上3个不同时间点每组取10只大鼠采集腹主动脉血后分离血清,对血清样本采用酶联免疫吸附试验(ELISA)测定动脉血相关指标脂多糖(LPS)、核转录因子-κB(NF-κB)、P53等的表达水平。结果与正常对照组比较,中暑模型组于制模后12、24、48 h三个时间点的体温、心率、LPS、NF-κB、P53均明显升高(P<0.05),MAP均明显降低(P<0.05);与模型组比较,ω-3鱼油脂肪乳治疗注射液组在制模后12、24、48 h三个时间点的如上动脉血相关指标均得到改善(P<0.05)。结论ω-3鱼油脂肪乳注射液可显著改善中暑大鼠的动脉血相关指标LPS、NF-κB、P53等的表达水平,提示其可抑制LPS进入血液、可能影响血管内皮细胞。Objective To investigate the effect of ω-3 fatty acids （Fat Emu Ision Injection） on factors in arterial blood and its mechanism. Methods Ninety SD rats were randomly divided into normal control group, heat stroke model group, heat stroke Omega-3 fish oil Fat Emulsion Injection treatment group, with 30 rats in each group. The rats were placed in the constant temperature box at 40 ℃ and 60% relative humidity for one hour to established heatstroke model. The Omega-3 fish oil fat emulsion injection treatment group received tail vein injection of Omega-3 fish oil Fat Emulsion 1.67 ml/（ kg· d） after molding. Normal control group and model group received the same dosage of normal saline of tail vein injection. One time per day for both group and continued for 3 days. The body temperature, heart rate, and mean arterial pressure （MAP） were recorded separately for 3 groups after 12 hours, 24 hours and 48 hours; At three different time point, abdominal aorta blood in 10 rats from each group w was drawn to prepare serum, and the levels of lipopolysac- charide （LPS）, nuclear factor kappa B （NF-kappa B） and P53 were tested by ELISA. Results Compared with normal control group, the heatstroke body temperature, heart rate, LPS, NF-B, P53 at three time points in model group were significantly increased （P 〈 . 05 ）, MAP decreased obviously （ P 〈 0. 05 ） ; compared with the model group, the above indexes in the Omega-3 fish oil fat emulsion injection treatment group at the three time points was improved obviously （P 〈 0. 05）. Conclusion Omega-3 fish oil fat emulsion injection can significantly improve the expression level of LPS, NF-kappa B and P53 in heat stroke rats, suggesting that it can inhibit LPS entry into blood and may affect vascular endo- thelial cells.

关 键 词：中暑大鼠模型 血管内皮细胞 ω-3鱼油脂肪乳注射液 动脉血相关指标 

分 类 号：R594.12[医药卫生—内科学]