蛇床子素对人精子细胞氧化应激损伤的保护作用  

Protective Effect of Osthole on Oxidative Stress Damage in Human Sperm Cells

作　　者：张光明[1] 杨锐[1] 毛骞[1] Zhang Guangming;Yang Rui;Mao Qian(Affiliated Hospital of Beihua University,Jilin 132011,China)

机构地区：[1]北华大学附属医院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2018年第3期338-341,共4页Journal of Beihua University（Natural Science）

基　　金：吉林省卫生技术创新项目(2017J076)

摘　　要：目的探讨蛇床子素对精子细胞氧化损伤的保护作用及机制.方法将收集的精子细胞分为5组,空白对照组无处理因素正常培养,模型组加入H2O2缺氧液培养1 h,各药物浓度组再加入H2O2缺氧液培养,同时加入50,150,450μmol/L蛇床子素培养液培养1 h,处理完毕后检测各组精子细胞存活率、精子活力、精子低渗膨胀实验、精子细胞SOD活力、MDA含量.结果蛇床子素可提高氧化损伤后精子存活率,增加精子活力,提高精子运动能力,提高精子细胞SOD活性,降低MDA含量,与模型组比较差异具有统计学意义(P<0.05).结论 50,150,450μmol/L蛇床子素均能减轻精子细胞缺氧损伤,其机制可能与蛇床子素能提高精子氧化损伤时细胞膜稳定性、保护细胞完整性、提高精子活力有关.Objective To investigate the protective effect and mechanism of osthole on the oxidative stress damage of spermatozoa. Method The sperm cells were collected and divided into five groups. The blank control group was cultured normally without treatment factors,and the model group was cultured with H2 O2 hypoxia solution for one hour. In each drug concentration group,H2 O2 anoxic solution and 50,150,450 μmol/L osthole medium were added at the same time,respectively,and were cultured for one hour. After treatment,the survival rate of sperm cells,sperm motility,hypotonic expansion of sperm tail test,SOD activity of spermatozoon,and MDA content were detected. Results Osthole increased the sperm survival rate,sperm motility and SOD activity,and reduced MDA content after oxidative stress damage. The differences among the experimental groups and the model group were significant（ P 0. 05）. Conclusion Osthole in the concentrations of 50,150 and 450 μmol/L can reduce the anoxia injury of sperm cells. The mechanism may be related to enhancing the membrane stability,protecting cell integrity and improving sperm motility.

关 键 词：蛇床子素 氧化损伤 精子活力 男性不育 

分 类 号：R285.5[医药卫生—中药学]