神经病理性疼痛大鼠中脑导水管周围灰质腹外侧区GABAAα_1受体的表达变化  

Changes of expression of GABAAα_1 receptor in ventrolateral periaqueductal gray in rats with neuropathic pain

作　　者：孙万秋[1] 王桂芝[1] 冀玉萍 李军[1] SUN Wanqiu;WANG Guizhi;JI Yuping;LI Jun(Department of Anesthesiology, Weifang Medical College, Weifang, 261053, Shandong Province, China)

机构地区：[1]潍坊医学院麻醉学系,山东潍坊261053

出　　处：《解放军医学院学报》2018年第4期332-334,352,共4页Academic Journal of Chinese PLA Medical School

基　　金：潍坊医学院科技创新基金(K1301020)

摘　　要：目的探讨γ-氨基丁酸A受体α_1(γ-aminobutyric acid type A receptor subunitα_1,GABAAα_1)在神经病理性疼痛(neuropathic pain,NP)大鼠中脑导水管周围灰质腹外侧区(ventrolateral periaqueductal gray,v LPAG)表达变化及其是否参与神经病理性疼痛表达过程。方法成年雄性清洁级SD大鼠15只,随机分为空白对照组(C组)、假手术组(S组)、神经病理性疼痛模型组(NP组)。建立坐骨神经分支选择性损伤(spared nerve injury,SNI)大鼠神经病理性疼痛模型,分别于术前1 d、术后1 d、3 d、5 d、7 d、14 d测定大鼠机械缩足反射阈值(paw withdrawal mechanical threshold,PWMT),采用蛋白免疫印迹法检测中脑导水管周围灰质腹外侧区处γ-氨基丁酸A受体α_1蛋白相对表达变化。结果与C组大鼠各时相机械缩足反射阈值比较,S组大鼠无明显改变。S组大鼠中脑导水管周围灰质腹外侧区处γ-氨基丁酸A受体α_1受体蛋白表达水平为0.699±0.027,较C组(0.636±0.023)无明显差异。NP组大鼠术后1 d机械缩足反射阈值为0.907±0.251 g,明显低于S组(8.216±1.428 g),并在后期呈持续性降低(P<0.05)。NP组大鼠中脑导水管周围灰质腹外侧区处γ-氨基丁酸A受体α_1受体蛋白相对表达量为1.442±0.015,明显高于S组(0.699±0.027)(P<0.05)。结论γ-氨基丁酸A受体α_1在神经病理性疼痛大鼠中脑导水管周围灰质腹外侧区表达明显上调,与机械缩足反射阈值呈负相关,可能与神经病理性疼痛相关。Objective To investigate the expression of GABAAα1 in the ventrolateral periaqueductal gray（v LPAG） and its involvement in neuropathic pain（NP） in rats. Methods Fifteen male SD rats were randomly divided into three groups： control group（group C）, sham operation group（group S） and neuropathic pain model group（group NP）. The model of neuropathic pain was established by using spared nerve injury（SNI）. The thresholds of mechanical contractions were measured at 1 day before operation and at 1, 3, 5, 7 and 14 days after operation paw withdrawal mechanical threshold（PWMT）. The relative expression of GABAAα1 receptor at v LPAG was detected by wetern blotting. Results Compared with the rats in group C, the rats in group S showed no significant changes in PWMT. The expression level of γ-aminobutyric acid A receptor α1 in the ventrolateral periaqueductal gray of the rats in group S was（0.699±0.027）, which was not significantly different from（0.636±0.023） in group C. In group NP, PWMT was（0.907±0.251） g at 1 day after operation, which was significantly lower than that of group S at（8.216±1.428） g and decreased at the later stage（P〈0.05）. The relative expression of γ-aminobutyric acid A receptor α1 in the ventrolateral periaqueductal gray of group NP was（1.442±0.015）, which was significantly higher than that of group S（0.699±0.027）（P〈0.05）. Conclusion The expression of GABAAα1 in v LPAG of rats with neuropathic pain up-regulates significantly, and it is negatively correlated with PWMT, which may be related to the pathogenesis of neuropathic pain.

关 键 词：神经病理性疼痛 γ-氨基丁酸A受体α1 中脑导水管周围灰质腹外侧区 

分 类 号：R338.2[医药卫生—人体生理学] R-332[医药卫生—基础医学]