基于CRISPR-Cas9的功能基因筛选研究进展  

Functional genetic screening using CRISPR-Cas9 system

作　　者：李欢欢 黄承浩 Huanhuan Li;and Chenghao Huang(National Institute of Diagnostics and Vaccine Development in b![~ctious Dciseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University, Xianwn 361102, Fujian, China)

机构地区：[1]厦门大学生命科学学院分子疫苗学和分子诊断学国家重点实验室国家传染病诊断试剂与疫苗工程技术研究中心,福建厦门361102

出　　处：《生物工程学报》2018年第4期461-472,共12页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.81571990)资助~~

摘　　要：功能性基因的筛选是探索生物进程、研究疾病发生发展和诠释基因功能的重要方法,在生物、医药、新治疗靶点筛选及肿瘤耐药等方面有广泛的应用。CRISPR-Cas9(Clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated protein 9)技术作为近期热门的基因编辑工具,能够高通量地对基因组进行精准修饰,为实现功能性基因的筛选提供了简便高效的技术支持。文中对CRISPR-cas9技术应用于功能性基因筛选的方法及研究进展进行了综述。Functional genetic screening as an important method for exploring biological processes, diseases development research and functional annotation of genetic elements, has been widely used in pharmaceutical research, new therapeutic targets identifying and screening, and tumor resistance. CRISPR-Cas9（Clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated protein 9） is the newest tool in the geneticist＇s toolbox, allowing researchers to edit genome with unprecedented ease, accuracy and high-throughput. CRISPR-Cas9 system provides a high-throughput, practical and efficient tool for the discovery of functionally important genes responsible for certain phenotypes. In this review, we summarize the characterization of CRISPR/Cas9 system and applications of this new genetic toolkit in functional genetic screening.

关 键 词：CRISPR-Cas9 基因敲除文库 功能性基因筛选 

分 类 号：Q78[生物学—分子生物学]