大鼠、豚鼠和金黄地鼠的尿液蛋白质组比较  

Comparison of urinary proteomes among rats, guinea pigs and golden hamsters

作　　者：倪娜 吴建强 李梨 高友鹤[3] Na Ni;Jianqiang Wu;Li Li;Youhe Gao(Institute of Molecular Medicine and Cancel, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China;Gene Engineering and Biotechnology Beijing Key Laboratory, Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing 100875, China;Department of Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences~Peking Union Medical College, Beijing 100005, China)

机构地区：[1]重庆医科大学基础医学院生物化学与分子生物学教研室分子医学与肿瘤研究中心,重庆400016 [2]中国医学科学院基础医学研究所/北京协和医学院基础学院病理生理学系,北京100005 [3]北京师范大学生物化学与分子生物学系北京基因工程与生物技术重点实验室,北京100875

出　　处：《生物工程学报》2018年第4期578-585,共8页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(No.2016YFC1306300); 国家重点基础研究发展计划(973计划)(No.2013CB530805); 北京自然科学基金(Nos.7173264,7172076); 北京师范大学基金(Nos.11100704,10300-310421102)资助

摘　　要：生物标志物是指与生理或病理变化相关的可监测的变化。尿液作为机体的一种排泄物,不受稳态机制的调节,可以反映机体的多种变化。动物模型可以模拟人类疾病过程,监测疾病的变化,并为早期诊断提供线索。大鼠作为常用的模型动物并非所有疾病的优势模型动物,因此比较大鼠与其他动物的尿液蛋白质组,从而为其他疾病选择优势模型动物提供线索。文中通过膜上酶切切成肽段再通过液相色谱与串联质谱偶联技术(LC-MS/MS)分析肽段信息,比较大鼠、豚鼠和金黄地鼠的尿液蛋白,结果显示3种鼠的尿蛋白数量不同,在机体不同系统中表达情况不同,参与的生物功能也不同。这为不同疾病选择不同的优势模型动物提供了依据。Biomarkers are the detectable changes associated with physiological or pathological changes. Urine as excreta ofthe body, without the mechanisms to maintain a homeostatic internal environment, can reflect a variety of changes in the body. Using animal models can simulate human disease processes, monitor disease changes, and provide clues to early diagnosis. Rats as commonly used model animals are not the dominant models for all disease, thus comparing the urinary proteins of rats with other animals to provide clues to the selection of other animal dominant models. In this study, urinary proteins were digested and profiled by liquid chromatography and tandem mass spectrometry（LC-MS/MS）. The urinary proteins of rats, guinea pigs and golden hamsters were compared. The results showed that the number of urine proteins in the three different animals was different, and also different in every system of the body. This provides a basis for selecting the best animal models for different diseases.

关 键 词：尿液蛋白质组学 液相色谱与串联质谱偶联技术 动物模型 大鼠 豚鼠 金黄地鼠 

分 类 号：R446.12[医药卫生—诊断学]