脂多糖对狼疮鼠骨髓幼红细胞巨噬细胞连接蛋白的影响  

The effects of lipopolysaccharide on expression levels of erythroblast macrophage protein in a mouse model of lupus

作　　者：王继辉[1] 凡慧 梁康礼[2] 范萍[2] 胡小倩[2] 陈金中[3] 程喜平[2] WANG Ji-hui;FAN hui;LIANG Kang-li;FAN Ping;HU Xiao-qian;CHEN Jin-zhong;CHENG Xi-ping(Department of Dermatology, the First Hospital of Foshan, Foshan 528000, Chin)

机构地区：[1]佛山市第一人民医院皮肤科,广东佛山528000 [2]广州医科大学附属第一医院皮肤科,广东广州510100 [3]复旦大学生命科学院遗传所遗传工程国家重点实验室,上海200433

出　　处：《临床皮肤科杂志》2018年第5期263-267,共5页Journal of Clinical Dermatology

基　　金：国家自然基金资助面上项目(81373649;81673983);广东省自然基金(2014A030313504)资助项目

摘　　要：目的:观察脂多糖(LPS)对狼疮鼠骨髓幼红细胞巨噬细胞连接蛋白(EMP)的影响。方法 :将狼疮鼠组(24只)分4组,分别腹腔注射不同浓度LPS(0、2、4及8 mg/kg);正常对照组(24只)分组和LPS处理方法同狼疮鼠组。采用定量聚合酶链式反应(PCR)检测骨髓EMP基因表达,免疫组化法观察骨髓中EMP阳性细胞。结果:(1)相同剂量LPS狼疮鼠组和正常对照组间比较:狼疮鼠组EMP m RNA表达比正常对照组显著性增高;(2)不同剂量LPS狼疮鼠组间比较:8 mg/kg LPS狼疮鼠组EMP m RNA表达高于2 mg/kg和0 mg/kg LPS狼疮鼠组;4 mg/kg和2 mg/kg LPS狼疮鼠组EMP表达较0 mg/kg LPS狼疮鼠组显著性增高;(3)不同剂量LPS正常对照组间比较:8 mg/kg LPS组EMP m RNA表达显著高于0 mg/kg LPS组;(4)免疫组化示EMP在狼疮鼠组及正常对照组中均有表达,细胞核、细胞质和细胞膜均被染色,以细胞质及细胞膜染色明显,随着LPS浓度增高,狼疮鼠组及正常对照组EMP阳性细胞更加密集。结论:EMP可能参与SLE发病,并与狼疮活动程度密切相关。Objective： To evaluate the effects of lipopolysaccharide（LPS） on expression levels of erythroblast macrophage protein （EMP） in a mouse model of lupus. Method： Twenty-four lupus mice were divided into 4 groups, and intraperitoneally injected with various concentrations of LPS（0 mg/kg, 2 mg/kg, 4 mg/kg and 8 mg/kg）. LPS-treated normal mice served as controls. Expres- sion levels of EMP mRNA in bone marrow were assessed using quantitative PCR while densities of EMP positive cells were e- valuated using immunohistochemieal staining. Result： ①Following LPS treatment, expression levels of EMP mRNA were signifi- cantly higher in lupus mice than in controls. ②In lupus mice, expression levels of EMP mRNA were significantly higher in mice treated with 8 mg/kg of LPS than in that treated with either 2 mg/kg of LPS or vehicle. In addition, both 4 mg/kg and 2 mg/kg of LPS significantly elevated expression levels of EMP mRNA in comparison with vehicle ③In comparison with vehicle treatment, 8 mg/kg of LPS also significantly increased expression levels of EMP mRNA in normal mice. ④Immunostaining results showed that EMP mainly expressed in the cytoplasm and cell membrane, but slightly expressed in cell nuclei, of both lupus and control mice. The den- sities of EMP positive cells in bone marrow were positively correlated with LPS concentrations. Conclusions： EMP could play a pathogenic role in SLE, and its expression is associated with the activity of lupus.

关 键 词：幼红细胞巨噬细胞连接蛋白 脂多糖 系统性红斑狼疮 

分 类 号：R593.24[医药卫生—内科学]