枣类甜蛋白基因家族的鉴定与生物信息学分析  

Identification and bioinformatics analysis of thaumatin-like protein gene in Chinese jujube

作　　者：刘潮[1] 韩利红[1] 王海波[1] 高永[1] 唐利洲[1] LIU Chao;HAN Lihong;WANG Haibo;GAO Yong;TANG Lizhou(Center for Yunnan Plateau Biological Resources Protection and Utilization. College of Biological Resource and Food Engineering. Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Pla- teau, Qujing Normal University, Qujing 655011, Yunnan, China)

机构地区：[1]曲靖师范学院云南高原生物资源保护与利用研究中心.生物资源与食品工程学院.云南省高校云贵高原动植物多样性及生态适应性进化重点实验室,云南曲靖655011

出　　处：《果树学报》2018年第4期393-401,共9页Journal of Fruit Science

基　　金：国家自然科学基金(31460179;31760103);云南省地方本科高校基础研究联合专项(2017FH001-034)

摘　　要：【目的】从枣全基因组中鉴定类甜蛋白(thaumatin like protein,TLP)基因,为枣TLP基因的功能研究与利用提供参考。【方法】通过生物信息学方法从枣基因组数据库中鉴定TLP基因家族成员,并对基因结构、进化、启动子区顺式作用元件和密码子使用性等进行分析。【结果】共鉴定到34个枣TLP基因,位于9条染色体上,分为4种结构类型,基因结构相对较简单;系统发育进化分析归为9个聚类组,其中聚类组5和聚类组6中的成员最多;基因启动子区发现多个与增强基因表达、激素响应和胁迫响应相关的元件;基因密码子使用偏性弱,基因进化主要受碱基突变的影响。【结论】枣基因组中含有34个TLP基因家族成员,数量相对偏少;在植物生长、果实发育和抵御胁迫过程中发挥作用;进化过程中主要受突变选择压力影响。该研究为TLP基因家族的功能分析和遗传育种应用提供了参考和借鉴。【Objective】Thaumatin like proteins（TLPs） include thaumatin, osmotin, zeamatin, and related proteins, which belong to the pathogenesis-related family（PR5） of plant defense proteins and are encoded by a multiple gene family. TLPs play an vital role in the enhancement of plant resistance to biotic and abiotic stresses due to involvement in defense system. Like other PRs, TLPs belong to a general plant stress response pathway rather than being specific to distinct stresses. TLPs possess antifungal,glucanase, allergens activities. Fruit diseases and storage would affect yield and fruit quality of Chinese jujube（Ziziphus jujuba Mill.）. A genome-wide identification was performed to investigate the TLP gene number, evolution and gene expression ability in Chinese jujube, through comparison with those of Arabidopsis thaliana, Oryza sativa and Populus trichocarpa.【Methods】TLP family member were identified based on the genome database of Chinese jujube. TLP sequences of A. thaliana were used as queries to obtain the member of the TLP family through searching the database of Chinese jujube protein on NCBI website. The sequences were then confirmed as encoding TLP for the presence of a thaumatin domain signature by SMART database searches. Chromosomal locations were determined using the annotation information of TLP genes genome data. The physiological and biochemical characteristics of protein were predicted using Prot Param at Ex PASy server. The schematic diagrams of the gene structure were drawn using Gene Structure Display Server. Multiple sequence alignments of full-length proteinsequences were accomplished using Clustal W. And the neighbor-joining（NJ） method in MEGA 5 software was used to carry out phylogenetic tree analyses of the Chinese jujube TLPs with the default evolutionary model, and bootstraps with 1 000 replicates were used to test the convergence of phylogenetic trees. The cis-acting elements distribution of the gene promoter was analyzed by Plant CARE database. Codon usage parameters

关 键 词：枣 类甜蛋白 生物信息学 密码子使用性 

分 类 号：S665.1[农业科学—果树学]