印楝素对黄粉虫幼虫呼吸作用和保护酶活性的影响  

Effects of Azadirachtin on Respiration and Protective Enzymes Activity of Tenebrio molitor Larvae

作　　者：郭春晖 宁昌龙 王蕊蕊 吴广 赵鹏飞 杨丙烨 杨振德[1] GUO Chun-hui;NING Chang-long;WANG Rui-rui;WU Guangl;ZHAO Peng-fei;YANG Bing-ye;YANG Zhen-de(College of Forestry, Guangxi University,Nanning 530001, China;Guangxi Liuwan State-owned Forest Farm, Yulin 537000, Guangxi, China;College of Plant Protection,Fujian Agriculture and Forest University,Fuzhou 350002,China)

机构地区：[1]广西大学林学院,南宁530001 [2]广西国有六万林场,广西玉林537000 [3]福建农林大学植物保护学院,福州350002

出　　处：《湖北农业科学》2018年第8期55-58,共4页Hubei Agricultural Sciences

基　　金：广西林业科技项目(桂林科字(2014)第25号)

摘　　要：为了解植物源农药对鞘翅目昆虫呼吸代谢和保护酶活性的影响,以黄粉虫(Tenebrio molitor)8龄幼虫为研究对象,以印楝素为供试农药,采用饲喂法测定印楝素对黄粉虫末龄幼虫CO2释放率和保护酶活性的变化。结果表明,印楝素处理后,黄粉虫的CO2释放率和保护酶活性均先升高后迅速下降。摄入印楝素6 h后,黄粉虫的CO2释放率达到最大值,为13.2μL/h,之后迅速下降并显著低于对照;保护酶SOD、CAT和POD活性在6 h时达到最大,分别是对照的2.34、2.91和1.98倍,其后呈现下降趋势。To investigate the effects of botanical pesticide on the respiration and protective enzymes of coleopteran,CO2 release rate and protective enzymes in the 8 th instar larvae of Tenebrio molitor fed with azadirachtin were examined. The results showed that the CO2 release rate and protective enzymes of T. molitor fed with azadirachtin increased initially and then declined rapidly. The highest CO2 release rate of T. molitor was 13.2 μL/h after 6 hours of feeding with azadirachtin and then fell quickly,remarkably lower than the value of control groups. The maximum activities of protective enzymes including SOD,CAT and POD appeared after 6 hours of feeding with azadirachtin, which were 2.34,2.91 and 1.98 times than those in the control group,respectively. And these protect enzymes lowered fast subsequently.

关 键 词：印楝素 黄粉虫(Tenebrio molitor) 呼吸 保护酶 

分 类 号：S433[农业科学—农业昆虫与害虫防治]