怀山药内生真菌的分离与鉴定  

Isolation and Identification of Endophytic Fungi from Dioscorea opposita Thunb

作　　者：文春南[1] 王晓龙[1] 李守婷 张朋展 叶颖晓 麻兵继[1] WEN Chun-nan;WANG Xiao-long;LI Shou-ting;ZHANG Peng-zhan;YE Ying-xiao;MA Bing-ji(College of Agronomy,Henan Agricultural University, Zhengzhou 450002, Chin)

机构地区：[1]河南农业大学农学院,郑州450002

出　　处：《湖北农业科学》2018年第8期58-60,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(U1604169);河南省教育厅科学技术研究重点项目(14A360006)

摘　　要：以怀山药(Dioscorea opposita Thunb)为试验材料,采用组织块分离法和研磨分离法对怀山药根茎中内生真菌进行分离,并运用形态学及分子生物学方法对其进行鉴定。结果表明,共分离得到23株真菌,经鉴定分别为镰孢菌属(Fusarium)、青霉属(Penicillium)、曲霉属(Aspergillus)、地霉菌属(Geotrichum)、弯孢菌属(Curvularia)和链格孢属(Alternaria)等6个属。The rhizome of Dioscorea opposita Thunb was used as the experimental material,and then endophytic fungi were isolated by the methods of tissue and grinding separation. The isolated strains were then identified by morphology and molecular biology approaches. The results showed that 23 strains from D. opposita Thunb were identified,which belonged to 6 genera,namely,Fusarium,Penicillium,Aspergillus,Geotrichum,Curvularia and Alternaria.

关 键 词：怀山药(Dioscorea opposita Thunb) 内生真菌 分子生物学方法 镰孢菌 

分 类 号：R284.2[医药卫生—中药学]