重楼活性单体pp-22对人鼻咽癌细胞CNE-1增殖、周期和凋亡的影响  

Effects of pp-22, a Monomer Purified from Paris Polyphylla on the Proliferation, Cycle and Apoptosis of Human Nasopharyngeal Carcinoma Cell CNE-1

作　　者：唐云云 谭桂香 蒋建伟[2] 林春兰[2] 严玉霞[2] 曾慧兰[3] 王新宁 王希成[1] TANG Yunyun;TAN Guixiang;JIANG Jianwei;LIN Chunlan;YAN Yuxia;ZENG Huilan;WANG Xinning;WANG Xicheng(The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China;Department of Biochemistry, Medical College, Jinan University, Guangzhou 510632, China;Department of Hematology, the First Affiliated Hospital of Jinan Uni- versity, Guangzhou 510632, China)

机构地区：[1]广东药科大学附属第一医院,广东广州510080 [2]暨南大学医学院生化系,广东广州510632 [3]暨南大学附属第一医院血液内科,广东广州510632

出　　处：《临床医学工程》2018年第4期441-443,共3页Clinical Medicine & Engineering

基　　金：广州市科技计划项目(201607010372);广东省中医药管理局建设中医药强省科研课题(2015118);暨南大学医学联合基金项目(21615465)

摘　　要：目的探讨重楼(Paris polyphylla)的醇提取物活性单体pp-22对人鼻咽癌CNE-1细胞的增殖、周期和凋亡的影响。方法采用MTT法,流式细胞术实验检测pp-22对人鼻咽癌CNE-1细胞的增殖、周期及凋亡的影响。结果重楼提取物活性单体pp-22能抑制CNE-1细胞的生长,并与时间、剂量呈正相关;流式细胞术检测显示,pp-22能导致人鼻咽癌CNE-1细胞发生G2/M期周期阻滞和凋亡。结论重楼醇提取物活性单体pp-22能够抑制CNE-1细胞增殖,引起G2/M期周期阻滞,诱导凋亡。Objective To investigate the effect ofpp-22, a monomer purified from Paris polyphylla on the proliferation, cycle and apoptosis of human nasopharyngeal carcinoma cell CNE-1. Methods MTT, flow cytometry were used to check the proliferation, cycle and apoptosis of CNE-1 cells after treatment with different concentrations ofpp-22. Results pp-22 inhibited CNE-1 cell proliferation in a concen- tration and time-dependent manner. Besides, pp-22 arrested CNE-1 cell cycle at G2/M phase and induced cell apoptosis. Conclusions The monomer purified from Pads polyphylla pp-22 can effectively inhibit cell proliferation and promote cell apoptosis in human CNE- 1 cells.

关 键 词：重楼单体 鼻咽癌 凋亡 

分 类 号：R285.5[医药卫生—中药学]