实验性家兔颈动脉粥样硬化模型中颈动脉I/M值与肾动脉粥样硬化的相关性研究  

Study on the correlation between the carotid I/M ratio and renal atherosclerosis in experimental rabbit carotid atherosclerosis models

作　　者：陈徐亮[1] 周野 杨鹏麟[1] CHEN Xuliang, ZHOU Ye, YANG Penglin(Department of Cardiovascular Medicine, the Second Hospital Affiliated to Wenzhou Medical University, Zhejiang Province, Wenzhou 325000, Chin)

机构地区：[1]温州医科大学附属第二医院心血管内科,浙江温州325000

出　　处：《中国医药导报》2018年第11期12-16,共5页China Medical Herald

基　　金：浙江省温州市科技计划项目(2013S0235)

摘　　要：目的分析实验性家兔颈动脉粥样硬化模型中颈动脉内膜/中膜(I/M)比值变化与肾动脉粥样硬化的相关性。方法将48只家兔以随机数字表法分为空白组、对照组、模型组,各16只。空白组不给予任何干预,维持喂饲基础饲料;对照组给予高脂饲料120g/d;模型组给予高脂饲料饲养7d后,于禁食(不禁水)12h后实施颈动脉内膜空气干燥术使其成为双侧颈动脉粥样硬化模型。获取所有家兔双侧手术段颈动脉、肾动脉标本,常规石蜡包埋、切片、HE染色后分析I/M值,并在实验结束时测定血清三酰甘油(TG)、总胆固醇(TC)、C反应蛋白(CRP)水平,分析尿微量白蛋白/尿肌酐(UACR)比值,并进行相关性分析。结果空白组家兔颈动脉、肾动脉光镜下HE染色正常;对照组颈动脉可见轻微内膜增生,肾动脉仅可见轻度增生狭窄,但无明显脂质沉积;模型组颈动脉内膜明显增生增厚,内膜下大量泡沫细胞堆积,肾动脉内膜广泛显著增生,血管内径严重狭窄,高倍镜下可见增厚内膜为泡沫细胞堆积。与空白组比较,对照组和模型组家兔颈动脉、肾动脉I/M值均明显升高,血清TG、TC、CRP及UACR均明显升高,且模型组家兔颈动脉、肾动脉I/M值及血清TG、TC、CRP、UACR均明显高于对照组,差异有统计学意义(P<0.05)。家兔颈动脉粥样硬化模型颈动脉I/M值与肾动脉I/M值、CRP、UACR呈正相关(P<0.05),与TG、TC相关性不明显(P>0.05)。结论颈动脉粥样硬化可促进肾动脉粥样硬化发生,可能与机体炎性反应、血管内皮细胞损伤、肾脏血管病变等有关。Objective To analyze the correlation between the change in carotid intima/media membrane（I/M） ratio and renal atherosclerosis in experimental rabbit carotid atherosclerosis models. Methods Forty-eight rabbits were randomly divided into the blank group, the control group and the model group by the random number table method, with16 rabbits in each group. The blank group was not given any intervention, which was given basal feed. The control group was given high-fat diet of 120g/d. The model group was given 7d of high-fat diet and then the rabbits were changed into bilateral carotid atherosclerosis models through carotid intima air drying after 12h of fasting（without fasting water）. The bilateral operative carotid arteries and renal artery specimens of all rabbits were obtained to analyze the I/M ratio after routine paraffin embedding, sectioning and HE stai ning. Serum levels of triglyceride（TG）, total cholesterol（TC） and C-reactive protein（CRP） were determined at the end of the experiment. Urinary microalbumin/urine creatinine（UACR） was analyzed and correlation analysis was performed. Results HE staining under light microscope was normal in the carotid artery and renal artery of rabbits in the blank group. In the control group, there was slight intimal hyperplasia in the carotid artery and slight hyperplasia and stenosis in the renal artery, without obvious lipid deposition. In the model group, there was obviously proliferated and thickened carotid intima, a large number of foam cells accumulated in the intima, extensively proliferated intima of the renal artery and seriously narrowed vessels,and thickened intima was accumulated foam cells at high magnification. Compared with the b lank group, the carotid artery, renal artery I/M ratio in the control group and model group were significantly increased, serum TG,TC, CRP and UACR were signi ficantly increased, and the carotid artery, renal artery I/M ratio and serum TG, TC, CRP and UACR in the model group were significantly higher than those

关 键 词：家兔 颈动脉粥样硬化 内膜/中膜比值 肾动脉粥样硬化 相关性 

分 类 号：R543.5[医药卫生—心血管疾病]