复合大孔聚多糖止血材料的制备及其体外性能研究  

Preparation of the macroporous polysaccharide hemostatic complex mate-rials and study on its performance in vitro

作　　者：许零[1] 马晓春[1] 曾莉君 陈妙源 王赟 居学成[1] 邱细妹 XU Ling1, MA Xiaochun1, ZENG Lijun2, CHEN Miaoyuan2 ,WANG Yun2, JU Xuecheng, QIU Ximei1,(1.Peking University Shenzhen Institute, Guangdong Province, Shenzhen 518057, China; 2.Xiamen Gelfeel Biological Technology Co., Ltd., Fujian Province, Xiamen 361026, Chin)

机构地区：[1]北京大学深圳研究院,广东深圳518057 [2]厦门凝赋生物科技有限公司,福建厦门361026

出　　处：《中国医药导报》2018年第11期17-20,共4页China Medical Herald

基　　金：广东省深圳市科技计划项目(JCYJ20150616163111759);福建省厦门市科技计划项目(3502Z20151087)

摘　　要：目的初步评价复合大孔聚多糖止血材料的止血效果,研究其体外性能。方法辐照交联法制备复合大孔聚多糖止血材料,通过红外及理化测试表征材料的结构、降解性能及凝血性能。结果止血材料经辐照后分子发生交联,吸水率达3456%;体外降解实验表明,止血材料降解性能良好,在溶菌酶溶液中3d即可降解68%,20d内则降解完全;材料的体外凝血时间为(25.33±2.45)s,能吸附更多的红细胞和血小板,血凝块强度更大。结论辐照交联技术制备的复合大孔聚多糖止血材料具有较好的吸液性能和凝血性能,在伤口止血方面具有良好的应用前景。Objective To evaluate the hemostatic effect of the macroporous polysaccharide hemostasis complex materials preliminarily, and to investigate the performance of the materials in vitro. Methods The hemostatic materials were prepared by γ-irradiation crosslinked method, and its structure, degradation in vitro and clotting performance were characterized by infrared spectrum and physicochemical test. Results The results showed that the crosslinked carboxymethylated-chitosan molecules had been made after the materials were irradiated, and its water absorption was of3456%. The biodegradable degradation of the materials in lysozyme solution was 68% after 3 days and the degradation completed within 20 days. The clotting time was（25.33±2.45）s, which indicated the material adhered more red blood cells and platelets and showed the stronger blood clots. Conclusion The macroporous polysaccharide hemostasis complex materials prepared by γ-irradiation crosslinked method possess great solution absorption performance and blood coagulation performance, which has good application prospect in wound hemostasis.

关 键 词：辐照交联 羧甲基壳聚糖 止血材料 体外降解 凝血 

分 类 号：R318.08[医药卫生—生物医学工程]