参萸补血方对再生障碍性贫血模型小鼠Th17细胞及其相关因子IL-6、IL-17及IL-23表达水平的影响  

Effects of Shenyu Buxue Decotion on the Th17 cells and expression of its related IL-6, IL-17 and IL-23 expression in mice with aplastic anemia

作　　者：曾清[1] 陆海颂[1] 程纬民[1] 何艾[1] 邓德君[2] 赖春凤 ZENG Qing;LU Haisong;CHENG Weimin;HE Ai;DENG Dejun;LAI Chunfeng(Department of Hematology, the First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 530023, China;Graduate School, Guangxi University of Chinese Medicine, Guangxi Zhuang Autonomous Region, Nanning 500001, China;Department of Oneology, Yullin Traditional Chinese Medicine Hospital, Guangxi Zhuang Autonomous Region, Yulin 537000, China)

机构地区：[1]广西中医学院第一附属医院血液科,广西南宁530023 [2]广西中医药大学研究生学院,广西南宁530001 [3]广西壮族自治区玉林市中医院肿瘤科,广西玉林537000

出　　处：《中国医药导报》2018年第12期13-16,共4页China Medical Herald

基　　金：广西自然科学基金项目(2013GXNSFAA019131)

摘　　要：目的观察参萸补血方对再生障碍性贫血(AA)模型小鼠Th17细胞及其相关因子白细胞介素6(IL-6)、IL-17及IL-23表达水平的影响,探讨其治疗AA的效果。方法选取清洁级BALB/c小鼠80只,按随机数字表法分为正常对照组、模型组、参萸补血方组、强的松(阳性对照)组。以^(60)CO-γ射线照射加腹腔注射环磷酰胺(CY)加氯霉素(CH)复合方法建立AA小鼠模型。造模成功后给药干预,连续4周后观察各组小鼠外周血象改变及各组小鼠Th17细胞及其相关因子IL-6、IL-17及IL-23表达水平的变化,光镜下观察骨髓增生程度。结果与正常对照组比较,模型组小鼠外周血象和骨髓增生明显降低,Th17细胞及其相关因子IL-6、IL-23和IL-17表达均显著升高,差异有统计学意义(P<0.05)。与模型组比较,参萸补血方组小鼠外周血像明显升高,Th17细胞及其相关因子IL-6、IL-23和IL-17表达水平下降,骨髓增生明显改善,差异有统计学意义(P<0.05)。结论参萸补血方可改善AA模型小鼠的骨髓造血功能,可能与其抑制T细胞免疫功能相关的IL-6、IL-17及IL-23表达水平有关。Objective To observe the effect of Shenyu Buxue Decotion on Th17 cells and the expression of IL-6, IL-17 and IL-23 in aplastic anemia（AA） model mice, and to explore its curative effect for AA. Methods Eighty clean-class BALB/c mice were divided into control group, model group, Shenyu Buxue Decotion group and Prednisone（positive control） group by random number table. The AA models were established by irradiated with60 CO-γ ray together with intraperitoneal injection of cyclophosphamide（CY） and chloramphenicol（CH）. After AA models were successfully established, mice were given to medicines for 4 weeks. The changes of mice peripheral hemogram, Th17 cells and the expression levels of IL-6, IL-17 and IL-23, and the degree of bone marrow hyperplasia under light microscope were ob served. Results Compared with the control group, the peripheral hemogram and bone marrow hyperplasia were decreased,while Th17 cells and expression of IL-6, IL-23 and IL-17 were increased in the model group, with statistically signifi cant difference（ P 〈 0. 05）. Compared with the model group, in the Shenyu Buxue Decotion group, the peripheral hemogram were increased, Th17 cells and the expression of IL-6, IL-23 and IL-17 were decreased, and bone marrow hyperplasia was improved, with statistically significant difference（P 〈 0.05）. Conclusion Shenyu Buxue Decotion can improve the bone marrow hematopoietic function of AA mice, which may due to the inhibition of the T cell immune function related IL-6, IL-17 and IL-23.

关 键 词：再生障碍性贫血 参萸补血方 TH17细胞 白介素17 白介素6 

分 类 号：R556.53[医药卫生—血液循环系统疾病]