Study on Extraction Process of Sipunculus nudus Polysaccharide and Its Antioxidant Activity  

作　　者：Wen CHEN Xiangjun WANG Dongxu SHAO Lirong LI Yuzhi HUANG Hongyuan SUN Lingyan LI Yong LIN wen chen;xiangjun wang;dongxu shao;lirong li;yuzhi huang;hongyuan sun;lingyan li;yong lin(School of Ocean Science and Technology,Hainan Tropic Ocean University Sanya 572022,china;School of Life Science and Ecology,Hainan Tropic Ocean University Sanya 572022,china)

机构地区：[1]school of ocean science and technology,hainan tropic ocean university,Sanya 572022,China [2]school of life science and ecology,hainan tropic ocean university,Sanya 572022,China

出　　处：《Agricultural Biotechnology》2018年第2期112-118,123,共8页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of Hainan Province(217157);Fund for Construction of Provincial Key Discipline(thalassochemistry)in 2017

摘　　要：[Objectives] This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide. [Methods]S. nudus polysaccharide was extracted by ultrasonic water extraction,with the marine S. nudus as a raw material from Sanya City,Hainan Province,by single factor variable method,to find the optimal extraction conditions including ultrasonic power,solid-liquid ratio,ultrasonic extraction temperature,ultrasonic extraction time,ultrasonic extraction times and lye concentration. The absorbance of the polysaccharide was determined by an ultraviolet spectrophotometer,and polysaccharide yield were calculated. The antioxidant activity of scavenging hydroxyl and DPPH radicals was measured at different concentrations. [Results]The optimal extraction conditions for S. nudus polysaccharide were as follows： solid-liquid ratio at 1∶11 mg/ml,extraction temperature at 60 ℃,three times of ultrasonic extraction,ultrasonic power of 960 W,extraction time of 1. 5 h,and lye concentration of 5%,under which the polysaccharide yield was 3. 21%. The scavenging rates of hydroxyl radical and DPPH radical were 12. 58% at the concentration of 0. 20 mg/ml. Moreover,the scavenging rates increased significantly with the increase of concentration. [Conclusions] This method is a new trial with satisfactory results.[Objectives] This study was conducted to optimize the extraction process of Sipunculus nudus polysaccharide. [Methods]S. nudus polysaccharide was extracted by ultrasonic water extraction,with the marine S. nudus as a raw material from Sanya City,Hainan Province,by single factor variable method,to find the optimal extraction conditions including ultrasonic power,solid-liquid ratio,ultrasonic extraction temperature,ultrasonic extraction time,ultrasonic extraction times and lye concentration. The absorbance of the polysaccharide was determined by an ultraviolet spectrophotometer,and polysaccharide yield were calculated. The antioxidant activity of scavenging hydroxyl and DPPH radicals was measured at different concentrations. [Results]The optimal extraction conditions for S. nudus polysaccharide were as follows： solid-liquid ratio at 1∶11 mg/ml,extraction temperature at 60 ℃,three times of ultrasonic extraction,ultrasonic power of 960 W,extraction time of 1. 5 h,and lye concentration of 5%,under which the polysaccharide yield was 3. 21%. The scavenging rates of hydroxyl radical and DPPH radical were 12. 58% at the concentration of 0. 20 mg/ml. Moreover,the scavenging rates increased significantly with the increase of concentration. [Conclusions] This method is a new trial with satisfactory results.

关 键 词：Sipunculus nudus Ultrasound Lye concentration Polysaccharide Antioxidant activityHome 

分 类 号：Q53[生物学—生物化学]