Polysaccharides from Huidouba and Its Effect on Type-2 Diabetes Mellitus  

作　　者：Chaoxi CHEN Meina XIE Zhihong HUANG 

机构地区：[1]College of Life Science and Technology,Southwest Minzu University,Chengdu 610041,China

出　　处：《Agricultural Biotechnology》2018年第2期156-159,共4页农业生物技术(英文版)

基　　金：Supported by the Funding from the Science and Technology Training Plan Project of Sichuan Province(2016KZ0007);Veterinary Medicine Discipline Program of Southwest University for Nationalities(2014XWD-S0906);the Fundamental Research Funds for the Central Universities of Southwest Minzu University(2017NZYQN38)

摘　　要：To evaluate the extraction process of Huidouba polysaccharides(HDBP)and its anti-diabetic activity,this study focused on the two contents as follows:extraction process of HDBP,which was optimized by orthogonal experiments using the extraction ratio of HDBP for the index;and bioactivity evaluation of HDBP on streptozotocin-induced diabetic rat model based on the histological,biochemical and enzyme linked immunosorbent assay(ELISA)method for histological observation,physiological and biochemical index such as fasting blood glucose(FBG),nitric oxide(NO),malondialdehyde(MDA),super oxide dismutase(SOD)and insulin level detection in pancreas tissue respectively.The results showed that HDBP extraction ratio was the highest under following conditions:the extraction temperature at 90℃,the extraction time of 1.5 h,and the solid-liquid ratio at 1:10.Compared with the normal control group and the model group,histological observation,physiological and biochemical index and insulin level in pancreas tissue significantly increased and FBG significantly decreased according to the dosage administrated.HDBP affected Streptozotocin-induced diabetic rats.

关 键 词：HDBP Diabetic rat Blood glucose Oxidative stressHome 

分 类 号：R285.5[医药卫生—中药学]