HepG2细胞中性红染色法测定化合物急性毒性的研究  

Acute toxicity determination of compounds by HepG2 cell neutral red staining

作　　者：徐剑 童晓玲 岑江杰[3] 纪磊[3] 林师道[3] 朱心强[1] XU Jian;TONG Xiao-ling;CEN Jiang-jie;JI Lei;LIN Shi-dao;ZHU Xin-qiang(School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China)

机构地区：[1]浙江大学医学院,浙江杭州310012 [2]金华市质量技术监督检测院 [3]浙江省化工研究院有限公司

出　　处：《预防医学》2018年第5期490-493,共4页CHINA PREVENTIVE MEDICINE JOURNAL

摘　　要：目的探索Hep G2细胞中性红染色法检测化合物毒性的可行性,为建立化合物急性毒性快速筛选方法提供依据。方法采用人肝癌Hep G2细胞中性红染色法检测秋水仙碱、环磷酰胺、吡蚜酮等30种化合物的细胞毒性IC50、IC45、IC40、IC35和IC30值。采用线性回归模型拟合ICx值对大鼠经口半数致死剂量(LD50)的回归方程,利用回归方程预测LD50值。采用LD50实际值和预测值对化合物毒性进行分级,比较两种方法的一致性。结果 lg(IC50)、lg(IC45)、lg(IC40)、lg(IC35)、lg(IC30)与lg(LD50)均有相关性(P<0.01),相关系数(r)为0.779~0.815;lg(IC50)与lg(LD50)的相关性最高,lg(LD50)=0.887×lg(IC50)+0.414。LD50实际值将30种化合物划分为高毒、中等毒和低毒各10种,LD50预测值划分出高毒12种、中等毒10种、低毒8种;两种方法的划分结果差异无统计学意义(P>0.05),一致率为86.7%。结论人肝癌Hep G2细胞中性红染色法可作为快速检测化合物急性毒性的方法。Objective To explore the feasibility of detecting compound toxicity in Hep G2 cell neutral red staining method and to provide the basis for the establishment of a rapid screening method. Methods The cytotoxic IC50,IC45,IC40,IC35 and IC30 values of 30 kinds of compounds,including colchicine,cyclophosphamide pipion and so on,were detected by the neutral red staining method of human liver cancer Hep G2 cells. The regression equation of the ICx value was used to predict the LD50 value of the rat via the linear regression method,and the LD50 was predicted by the regression equation with the highest correlation.The toxicity of the compound was graded by the actual value and predicted value of LD50,and the consistency of the two methods was compared. Results The correlation between lg（IC50）,lg（IC45）,lg（IC40）,lg（IC35）,lg（IC30） and lg（LD50）was statistically significant（P〈0.01）,and correlation coefficient（r） was 0.779-0.815. The correlation between lg（IC50） and lg（LD50） is the highest,lg（LD50） =0.887×lg（IC50）＋ 0.414. The actual value of LD50 divided 30 compounds into 10 kinds of high toxicity,medium toxicity and low toxicity,and the predicted value of LD50 was divided into 12 types of high toxicity,10 medium toxicity and 8 low toxicity. There was no significant difference between the two methods（P〉0.05）,and the concordant rate was 86.7%. Conclusion The cell neutral red staining method can be used as a method for rapid detection of acute toxicity.

关 键 词：急性毒性 HEP G2细胞 中性红染色法 

分 类 号：R99[医药卫生—毒理学]