HGF转染的骨髓间充质干细胞移植在输尿管部分梗阻大鼠肾脏中的转化  

Research of mesenchymal stem cells transfected with hepatocyte growth factor transplantation in the rat＇s kidney with partial unilateral ureteral obstruction

作　　者：薛彦生[1] 刘鑫[1] 赵琦[1] 杨屹[1] Xue Yansheng;Liu Xin;Zhao Qi;Yang Yi(Department of Pediatric Urology, Shengjing Hospital of China Medical University, Shenyang 110004, China)

机构地区：[1]中国医科大学附属盛京医院小儿泌尿外科,沈阳110004

出　　处：《国际儿科学杂志》2018年第4期307-310,F0003,共5页International Journal of Pediatrics

基　　金：国家自然科学基金项目（81571514）

摘　　要：目的观察移植的肝细胞生长因子修饰的骨髓间充质干细胞在输尿管部分梗阻鼠肾脏内的转化情况。方法体外分离培养雄性大鼠骨髓间充质干细胞，并转染肝细胞生长因子；同时选择成年健康雌性SD大鼠36只，数字表法随机分为对照组（A）、实验组B和实验组C，各12只，制作单侧输尿管部分梗阻模型，术后7d解除梗阻。对梗阻侧肾脏实质进行多点注射：A组单纯注射生理盐水，B组单纯注射骨髓间充质干细胞，C组注射肝细胞生长因子修饰的骨髓间充质干细胞。于注射术后7 d，处死3组大鼠，利用免疫荧光方法检测骨髓间充质干细胞在输尿管部分梗阻大鼠肾脏中的分布及转化情况。结果在输尿管部分梗阻大鼠解除梗阻术后7 d，梗阻侧肾脏肾小管上皮细胞内可见绿色荧光标记的骨髓间充质干细胞；部分移植的骨髓间充质干细胞发生了细胞表型转化，表达肾小管上皮细胞标志物；C组与B组相比，移植后存活的骨髓间充质干细胞数量和发生细胞表型转化骨髓间充质干细胞数量明显增高（P〈0.05）。结论在输尿管部分梗阻大鼠模型中，在肾脏实质注射骨髓间充质干细胞可以在局部存活，并分化为肾小管上皮细胞；肝细胞生长因子有促进骨髓间充质干细胞存活和分化的可能。Objective To observe the phenotypic transformation of bone marrow mesenchymal stem cells （MSC）transfected with hepatocyte growth factor （HGF）after their transplantation in the rat＇s kidney with partial unilateral ureteral obstruction （PUUO）. Methods We isolated, cultured bone MSCs of the male rats, and transfected them with Ad-HGF in vitro. Thirty-six female rats with PUUO were randomly divided into control group （A） and the experimental groups （ B, C）. Saline, Ad-GFP transfected MSCs or Ad-HGF transfected MSCs were respectively injected into the parenchyma of kidney on the 7th day with PUUO. We released the ureteral obstruction of rats after transplantation. Kidney tissue of the rats were collected on the 7th day after transplantation. The distribution and phenotypic transformation of MSCs in the kidney were determined by method. Results The green fluorescent-labeled MSCs mainly distributed in the tubular cells, and a part of bone MSCs underwent phenotypic transformation after transplantation. Compared with group B, the number of bone MSCs with phenotypic transformation significantly increased in group C. Conclusion After transplantation, MSCs can survive and differentiate into renal tubular epithelial cells, and HGF may promote survival and differ- entiation of MSCs.

关 键 词：骨髓间充质干细胞 输尿管部分梗阻 肝细胞生长因子 大鼠 

分 类 号：R726.9[医药卫生—儿科]