HPLC法同时测定锥叶柴胡中6个主要皂苷类成分的含量  

HPLC simultaneous determination of 6 main saponins in Bupleurum bicaule Helm

作　　者：魏晓萌 严华[2] 陆以云 麻思宇 程显隆[2] 魏锋[2] 杜树山[3] WEI Xiao-meng;YAN Hua;LU Yi-yun;MA Si-yu;CHENG Xian-long;WEI Feng;DU Shu-shan(Faculty of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China;National Institutes for Food and Drug Control, Beijing 100050, China;Beijing Key Laboratory of Traditional Chinese Medicine Protection and Utilization, Beijing Normal University, Beijing 100875, China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]中国食品药品检定研究院,北京100050 [3]北京师范大学中药资源保护与利用北京市重点实验室,北京100875

出　　处：《药物分析杂志》2018年第4期618-622,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:采用HPLC法同时测定锥叶柴胡中6个皂苷类成分柴胡皂苷A、C、D及柴胡次皂苷F、G、I的含量。方法:采用Inert Sustain AQ-C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相进行梯度洗脱,流速1.0 m L·min^(-1),检测波长210 nm,柱温25℃。结果:柴胡皂苷A、C、D及柴胡次皂苷F、G、I质量浓度分别在8.32~208、3.50~88.2、24.0~600、13.2~331、31.3~783、6.65~166μg·m L^(-1)范围内呈良好的线性关系(r≥0.999);平均回收率为91.1%~107.7%(RSD<3%,n=6)。12批样品中上述6个成分含量分别为0.11%~0.34%、0.05%~0.20%、0.35%~1.92%、0.13%~0.87%、0.18%~1.49%、0.04%~0.37%。结论:新建立的含量测定方法准确可靠,可用于锥叶柴胡的质量控制。Objective：To establish an HPLC method for simultaneous determination of saikosaponin A,saikosaponin C,saikosaponin D,prosaikogenin F,prosaikogenin G and prosaikogenin I in Bupleurum bicaule Helm.Methods：HPLC analysis was performed on an Inert Sustain AQ-C18 column（4.6 mm×250 mm,5 μm） with a gradient elution of acetonitrile and water.The flow rate was 1.0 mL·min^-1,the detection wavelength was 210 nm and the column temperature was 25 ℃.Results：The linear ranges of saikosaponin A,saikosaponin C,saikosaponin D,prosaikogenin F,prosaikogenin G and prosaikogenin I were 8.32-208 μg·mL^-1,3.50-88.2 μg·mL^-1,24.0-600 μg·mL^-1,13.2-331 μg·mL^-1,31.3-783 μg·mL^-1,6.65-166 μg·mL^-1,respectively.The average recoveries were 91.1%-107.7%（RSD〈3%,n=6）.The contents of the six components in 12 samples of B.bicaule were 0.11%-0.34%,0.05%-0.20%,0.35%-1.92%,0.13%-0.87%,0.18%-1.49%,0.04%-0.37%,respectively.Conclusion：The newly established method is accurate and reliable,which can be used for the quality control of B.bicaule.

关 键 词：锥叶柴胡 柴胡皂苷 柴胡次皂苷 含量测定 质量控制 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]