高效液相色谱法同时测定软肝缩脾丸中7个成分的含量  

Simultaneous determination of seven constituents in Ruangan Suopi pills by HPLC

作　　者：华之卉[1] 刘栋[2] 李明春[3] HUA Zhi-hui;LIU Dong;LI Ming-chun(Dept. of Pharmacy, Zhengzhou People＇ s Hospital, Zhengzhou 450003, China;Dept. of Preparation, No. 153 Central Hospital of PLA, Zhengzhou 450042, China;Dept. of Pharmacy, No. 401 Hospital of PLA, Qingdao 266071, China)

机构地区：[1]郑州人民医院药学部,郑州450003 [2]解放军第153中心医院制剂室,郑州450042 [3]解放军第401医院药剂科,青岛266071

出　　处：《药物分析杂志》2018年第4期703-709,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：军队医疗机构制剂标准提高科研专项课题(No.14ZJZ02)

摘　　要：目的:建立高效液相色谱法同时测定软肝缩脾丸中芍药苷、丹酚酸B、大黄酸、五味子醇甲、大黄素、大黄酚、丹参酮ⅡA 7个有效成分的含量。方法:采用Wondasil C18色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长230 nm(0~34.0 min,检测芍药苷、丹酚酸B、大黄酸)、217 nm(34.0~37.0 min,检测五味子醇甲)、225 nm(37.0~56.0 min,检测大黄素、大黄酚)、270 nm(56.0~60.0 min,检测丹参酮ⅡA)。结果:芍药苷、丹酚酸B、大黄酸、五味子醇甲、大黄素、大黄酚、丹参酮ⅡA的线性范围分别为10.04~250.95μg·mL^(-1)(r=0.999 5)、10.25~256.28μg·mL^(-1)(r=0.999 7)、0.84~21.07μg·mL^(-1)(r=0.999 5)、0.91~22.86μg·mL^(-1)(r=0.999 2)、0.67~16.72μg·mL^(-1)(r=0.999 1)、1.45~36.30μg·mL^(-1)(r=0.999 5)、0.38~9.40μg·mL^(-1)(r=0.999 3);平均加样回收率(n=9)为99.5%~101.6%,RSD为0.61%~1.1%。样品中上述7个有效成分的含量范围分别为2.327~2.835、2.423~3.158、0.051~0.207、0.052~0.326、0.089~0.155、0.330~0.481、0.045~0.087 mg·g-1。结论:本法可用于软肝缩脾丸中芍药苷、丹酚酸B、大黄酸、五味子醇甲、大黄素、大黄酚、丹参酮ⅡA 7个有效成分的含量测定。Objective：To establish an HPLC method for simultaneous determination of paeoniflorin,salvianolic acid B,rhein,schisandrin,emodin,chrysophanol,and tanshinone ⅡA in Ruangan Suopi pills.Methods：The chromatographic separation was performed on a Wondasil C18 column（250 mm×4.6 mm,5 μm）at 30 ℃ with acetonitrile（A）-0.1% phosphoric acid solution（B）as mobile phase in a gradient elution.The flow rate was 1.0 mL·min^-1 and the UV detection wavelength was set at 230 nm（0-34.0 min for paeoniflorin,salvianolic acid B and rhein）,at 217 nm（34.0-37.0 min for schisandrin）,at 225 nm（37.0-56.0 min for emodin and chrysophanol）,and at 270 nm（56.0-60.0 min for tanshinone ⅡA）.Results：The linear ranges of paeoniflorin,salvianolic acid B,rhein,schisandrin,emodin,chrysophanol,and tanshinone ⅡA were 10.04-250.95 μg·mL^-1（r=0.999 5）,10.25-256.28 μg·mL^-1（r= 0.999 7）,0.84-21.07 μg·mL^-1（r=0.999 5）,0.91-22.86 μg·mL^-1（r=0.999 2）,0.67-16.72 μg·mL^-1（r=0.999 1）,1.45-36.30 μg·mL^-1（r=0.999 5）,0.38-9.40 μg·mL^-1（r=0.999 3）,respectively.The average recoveries（n=9）were 99.5%-101.6% with RSD of 0.61%-1.1%.The contents of above-mentioned seven constituents in samples were 2.327-2.835 mg·g^-1,2.423-3.158 mg·g^-1,0.051-0.207 mg·g^-1,0.052-0.326 mg·g-1,0.089-0.155 mg·g^-1,0.330-0.481 mg·g^-1,0.045-0.087 mg·g^-1,respectively.Conclusion：The method can be used to determine the content of paeoniflorin,salvianolic acid B,rhein,schisandrin,emodin,chrysophanol,and tanshinone ⅡA in Ruangan Suopi pills.

关 键 词：软肝缩脾丸 赤芍 丹参 大黄 五味子 芍药苷 丹酚酸B 大黄酸 五味子醇甲 大黄素 大黄酚 丹参酮ⅡA 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]