应用基质辅助激光解析电离飞行时间质谱鉴定假丝酵母菌  

Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry todifferentiate Candida

作　　者：杨静 石玉[1] 樊尚荣[1] 朱玉霞[1] 刘小平[1] Yang Jing;Shi Yu;Fan Shangrong;Zhu Yuxia;Liu Xiaoping(Depatment of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen 518036, China)

机构地区：[1]北京大学深圳医院妇产科,518036

出　　处：《中华检验医学杂志》2018年第4期296-299,共4页Chinese Journal of Laboratory Medicine

摘　　要：目的评价应用基质辅助激光解析电离飞行时间质谱（MALDI－TOF－MS）对外阴阴道假丝酵母菌病（VVC）的假丝酵母菌鉴定的准确性。方法应用分子方法包括PCR基因扩增和测序分析鉴定WC的假丝酵母菌，应用MALDI－TOF－MS鉴定分子方法确认的假丝酵母菌，建立白假丝酵母菌复合体鉴定数据库。结果从VVC标本中应用分子方法鉴定出包括白假丝酵母菌、非洲假丝酵母菌、都柏林假丝酵母菌、光滑假丝酵母菌、C.bracarensis、C.nivariensis、季也蒙假丝酵母菌、葡萄牙假丝酵母菌、克柔假丝酵母菌、乳酒假丝酵母菌、近平滑假丝酵母菌、C.metapsilosis、热带假丝酵母菌、奥默柯达菌、费比恩毕赤酵母菌、胶红酵母菌、酿酒酵母菌、阿萨希丝孢酵母等在内的假丝酵母菌324株，应用MALDI－TOF－MS能够正确鉴定株314株，正确鉴定率为96.91%，3株白假丝酵母菌、1株非洲假丝酵母菌、1株光滑假丝酵母菌、2株C.metapsilosis、1株季也蒙假丝酵母菌和1株酿酒酵母菌鉴定错误。1株Torulaspora pretoriensis未能鉴定。应用自建白假丝酵母菌复合体数据库可正确鉴定白假丝酵母菌153株（98.08%）、非洲假丝酵母菌37株（97.37%）和都柏林假丝酵母菌1株（100%），总正确鉴定率为97.95%（191/195）。结论MALDI－TOF－MS可用于快速和准确鉴定假丝酵母菌，对假丝酵母菌复合体的鉴定有优势。Objective To evaluate the efficiency of matrix assisted laser analytical ionization time of flight mass spectrometry （MALDI-TOF-MS） for identification of Candida species which caused vulvovaginal candidiasis（VVC）. Methods Candida strains from VVC were first identified by PCR amplification of the ITS regions and transposable intron of DNA and sequencing analysis. MALDI-TOF-MS wasfurther performed to identify the strainsconfirmedby molecular methods, and at the same time the MALDI-TOF-MS identification database of C. albieans complex was set up. Results In total, 324 Candida strains were identifiedby molecular methods from VVC samples, which encompassed20 different yeast species, including C. albieans, C. africana, C. dubliniesis, C. g｜abrata, C. bracarensis, C. nivariensis, C. guilliermondii, C. lusitaniae, C. krusei, C. kefyr, C. parapsilosis, C. metapsilosis, C. tropicalis, Kodamaea ohmeri, C. fabianii, Rhodotorula mucilaginosa, Saccharomyces cerevisiae, and Trichosporon asahii. 314of all 324 Candida strains were correctly identified by MALDI-TOF-MS. 3 C. albieans, 1 C. africana, 1 C. glabrata, 2 C. metapsilosis, 1 C. guilliermondii and 1 Saecharomyces cerevisiae were incorrectly identified, while 1 Torulaspora pretoriensiscannot identified. The accuracy ofidentification was 96. 91% （314/324）. 97.95% （191/195）of Candida strains, including 153 （98.08%） C. albicans, 37 （97.37%） C. africanaand 1 （100%） C. dubliniesis, can be correctly identifiedby the newbuiltC, albicans complex database. Conclusion MALDI-TOF-MS is proved to he a rapid and reliable method for identification of Candida strains from VVC, which also has advantages of identification of Candida complex.

关 键 词：假丝酵母菌病 外阴阴道 假丝酵母菌属 光谱法 质量 基质辅助激光解吸电离 

分 类 号：R446.5[医药卫生—诊断学]