温度对ASBBR处理榨菜废水及微生物种群的影响  

Effect of Temperature on Performance and Microbial Population of Pilotscale ASBBR Treating Hypersaline Mustard Tuber Wastewater

作　　者：温宇惠 林子源 王佳乐[1] 张永胜[1] 何磊 周健[1] WEN Yu-hui;LIN Zi-yuan;WANG Jia-le;ZHANG Yong-sheng;HE Lei;ZHOU Jian(Key Laboratory of Three Gorges Reservoir Region＇ s Eco - Environment 〈 Ministry of Education 〉 , Chongqing University, Chongqing 400045, Chin)

机构地区：[1]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《中国给水排水》2018年第9期20-25,共6页China Water & Wastewater

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07315-004)

摘　　要：以超高盐(以Na Cl计为70 g/L)榨菜废水为研究对象,通过中试考察温度对ASBBR反应器处理效能及微生物种群的影响。结果表明,季节温度变化对系统去除COD的效能影响显著。当负荷为3 kg COD/(m^3·d)时,反应器在夏季(26~39℃)、秋季(13~25℃)、冬季(3~12℃)的COD平均去除率分别为89.43%、78.51%、66.35%。PCR-DGGE分析结果表明,超高盐榨菜废水处理系统的微生物种群与接种污泥相比发生了显著变化;温度对系统微生物种群结构及优势微生物种群影响显著,微生物种群丰富度呈现出夏季>秋季>冬季的规律,夏季与秋季、冬季的微生物种群相似性分别为49.6%、45.2%;根据16S r DNA V3区测序结果,系统优势微生物种群主要为厌氧嗜盐菌,温度对有机物降解优势微生物功能菌属影响显著,夏季系统中主要有Marinobacterium、Lutaonella、Cellulophaga、Arcobacter、Methanobacterium,冬季系统主要有Marinobacterium、Arcobacter、Methanobacterium。Using 70 g/L NaCl pickle mustard wastewater as influent, pilot-scale anaerobic sequencing batch biofilm reactor （ASBBR） was conducted over 300 days to study the effect of seasonal change on performance and microbial community structure. Results showed that the temperature variation had a significant influence on the COD removal efficiency. When the organic loading rate was 3 kgCOD/（ m3 d） , the COD removal efficiencies of ASBBR were 89.43% , 78.51% and 66.35% respectively at summer, autumn and winter. The results of PCR-DGGE showed that the microbial population of hypersaline wastewater biological treatment system changed remarkably from seed sludge, and the temperature had a huge impact on microbial composition. The microbial population richness decreased with the seasonal change from summer to winter, dominant bacterium showed seasonal differences. The Dice coefficients of summer with autumn and winter were 49.6% and 45.2%. The results of 16S rDNA V3 region sequencing technology indicated that the anaerobic halophilic bacteria were the dominant microbial populations in the saline wastewater treatment system. In summer, the predominant pollutant-degrading halophilic bacteria belonged to Marinobacterium, Lutaonella, Cellulophaga, Arcobacter and Methanobacterium. In winter, the predominant species belonged to Marinobacterium,Arcobacter and Methanobacteriurn.

关 键 词：超高盐榨菜废水 ASBBR 中试 微生物种群 温度 

分 类 号：TU992[建筑科学—市政工程]