低糖野生种与高糖栽培种西瓜果实代谢产物组分差异分析  

Variance Analysis of Metabolite Components Between Low Sugar Wild and High Sugar Cultivated Watermelon Fruits

作　　者：崔霞霞 王亚钦[2] 任毅[2] Alisdair R Fernie Saleh Alseekh 何洪巨[2] 宫国义[2] 张海英[2] 郭绍贵[2] 张洁[2] 许勇[2] CUI Xiaxia;WANG Yaqin;REN Yi;Alisdair R.Fernie;Saleh Alseekh;HE Hongju;GONG Guoyi;ZHANG Haiying;GUO Shaogui;ZHANG Jie;XU Yong(Plant Science and Technology College, Beo＇ing University of Agriculture, Beijing 102206, China;National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops （North China）, Ministry of Agriculture, Beijing Vegetable Germplasm Improvement Lab, Beijing 100097, China;Max_Planck_lnstitute of Molecular Plant Physiology, Potsdam 14476, Germany;Center of Plant System Biology and Biotechnology, Plovdiv 4000, Bulgaria)

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,北京蔬菜种质改良实验室,北京100097 [3]马克斯.普朗克分子植物生理研究所,德国波茨坦14476 [4]植物系统生物学和生物技术研究中心,保加利亚普罗夫迪夫4000

出　　处：《园艺学报》2018年第4期775-783,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金面上项目(31772328); 北京市科技新星项目(Z171100001117032); 国家‘十三五’重点研发项目(2016YFD0100506); 北京市创新团队项目(BAIC10-2017)

摘　　要：采用高效液相色谱—串联质谱(UHPLC–MS/MS)技术对西瓜5份具有代表性的材料果实进行代谢产物分析,在此基础上选取栽培西瓜高糖材料‘97103’和野生西瓜低糖材料‘PI296341’,利用气相色谱—质谱联用(GC–MS)方法对主代谢产物进行定量分析。从成熟果实中定量鉴定出58种代谢物,其中22种在‘97103’和‘PI296341’间存在显著差异。韧皮部运输的棉籽糖在野生低糖材料果实中大量滞留,而在栽培高糖材料中极少积累。综合差异代谢物及其代谢途径分析发现,高糖材料‘97103’果实在水苏糖、棉籽糖等光合产物卸载途径及蔗糖积累代谢途径上的代谢产物积累较高;而低糖材料‘PI296341’,则在氨基酸代谢、有机酸代谢途径及6–磷酸葡萄糖等糖代谢途径上产物积累较高。这些发现为野生西瓜进化到栽培种果实品质的代谢产物积累和调控提供了重要依据,也可为提高西瓜品质提供指导。Five representative watermelon accessions were analyzed using UHPLC–MS/MS,and the primary metabolites of the high sugar cultivar‘97103＇and low sugar wild accession‘PI296341＇fruits were quantified using Gas Chromatography–Mass Spectrometery（GC–MS）. A total of 58 metabolites were identified,among which 27 compounds were significantly different. Raffinose content was much higher in the wild accession PI296341 than that in the cultivar 97103 fruits,and very few raffinose remained in the cultivar 97103 fruits. Analysis of differential metabolites and metabolic pathways revealed that cultivar 97103 fruits accumulated more metabolites from photosynthate unloading（raffinose and stachyose）and sucrose accumulating pathways,while the metabolites from the metabolism pathways of amino acids and organic acids and glucose-6-phosphate were the majority in PI296341 fruits. These findings provide important insights into the metabolic evolution from wild to cultivated species. The identification of metabolic properties can provide technical guidance for future improvement of watermelon quality.

关 键 词：西瓜 代谢组学 光合产物卸载 蔗糖积累 糖代谢途径 

分 类 号：S651[农业科学—果树学]