木薯内源ABA含量与块根淀粉积累关系研究  

The Relationship Between Endogenous Abscisic Acid Content and Starch Accumulation in Root Tuber of Cassava

作　　者：罗兴录[1,2] 潘晓璐 朱艳梅 LUO Xinglu;PAN Xiaolu;ZHU Yanmei(Agricultural College of Guangxi University, Nanning, Guangxi 530005, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourees, Nanning, Guangxi 530004, China)

机构地区：[1]广西大学农学院,广西南宁530005 [2]亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004

出　　处：《热带作物学报》2018年第3期472-479,共8页Chinese Journal of Tropical Crops

基　　金：亚热带农业生物资源保护与利用国家重点实验室开放课题(No.SKLCDSA-b201609);广西科学研究与技术开发计划项目(桂科重14121005-2-1);国家现代农业产业体系广西薯类产业创新团队"薯类产业功能专家"(No.nycytxgxcxtd-03-11-2);南宁市科学研究与开发计划项目(No.201109044B)

摘　　要：以淀粉含量不同的木薯品种‘辐选01’和‘华南124’为材料,通过测定木薯内源脱落酸(ABA)含量、淀粉积累过程中的关键酶活性以及淀粉积累量,探讨ABA含量与块根淀粉积累的关系。结果表明:不同淀粉含量的木薯品种其内源ABA含量存在差异,尤其在生育后期表现尤为明显;淀粉含量高的品种叶片、茎秆和块根的ABA含量、块根腺苷二磷酸葡萄糖焦磷酸化酶(AGPpase)、蔗糖磷酸合成酶(SPS)、可溶性淀粉合成酶(SSS)及淀粉分支酶(SBE)的活性高于淀粉含量低的品种;结果表明,叶片ABA含量与块根的腺苷二磷酸葡萄糖焦磷酸化酶(AGPase)、可溶性淀粉合成酶(SSS)及淀粉分支酶(SBE)活性呈极显著正相关,与蔗糖磷酸合成酶(SPS)活性呈显著正相关。茎秆ABA与腺苷二磷酸葡萄糖焦磷酸化酶(AGPpase)呈显著正相关,与可溶性淀粉合成酶(SSS)以及淀粉分支酶(SBE)的活性呈极显著正相关;块根ABA含量与块根腺苷二磷酸葡萄糖焦磷酸化酶(AGPpase)、可溶性淀粉合成酶(SSS)活性呈极显著正相关,与蔗糖磷酸合成酶(SPS)活性呈现正相关,与块根淀粉分支酶(SBE)活性呈极显著负相关。茎叶、块根ABA含量与块根淀粉积累均呈现极显著正相关。可见,木薯内源ABA在块根淀粉积累中有重要调节作用。本研究为高产、高淀粉木薯品种的选育与栽培提供理论支持和实践指导。The relationship between endogenous abscisic acid and starch accumulation in the root tuber of cassava was studied by measuring abscisic acid content, key enzymes activities and starch content in the process of starch accumulation with different starch content cassava cultivars RS01 and SC124 as the materials. The results showed that the content of ABA in the cassava cultivars with different starch contents was different, especially in the late growth stage. The ABA content of leaves, stems and roots in the starch content was higher than that in the control group （ADPGpase） （p 〈0.05）. The activities of sucrose phosphate synthase （SPS）, soluble starch synthase （SSS）and starch branching enzyme were higher than those with low starch content. The results showed that the content of ABA in leaves was different from that of adenosine diphosphate glucose pyrophosphorylase（p〈0.05）, and the activities of soluble starch synthase（SSS）and starch branching enzyme（SBE）were significantly correlated with sucrose phosphate synthase（SPS）activity. There was a significant positive correlation between ABA and ADPGpase, and there was a significant positive correlation with the activity of soluble starch synthase （SSS）and starch branching enzyme（SBE）. The ABA content of roots was similar to that of tuberous gland（p〈0.05）, and it was positively correlated with the activities of sucrose phosphate synthase（SPS）, and the activities of starch-branched starch synthase（SBE）were extremely significantly negatively correlated. There was a significant positive correlation between ABA content and root starch accumulation. It can be seen that cassava endogenous ABA plays an important role in the accumulation of starch in the roots.

关 键 词：木薯 内源ABA 淀粉合成关键酶 淀粉积累 

分 类 号：S533[农业科学—作物学]