巴西橡胶树叶肉细胞原生质体中细胞骨架结构及动态观察  

Visualization of Cytoskeleton Organization and Dynamics in the Rubber Tree (Hevea brasiliensis) Mesophyll Protoplasts

作　　者：王倩男 张晓东 罗红丽 安邦 WANG Qiannan;ZHANG Xiaodong;LUO Hongli;AN Bang(Haintrn Key Laboratory for Sustainable Utilization of Tropical Bioresources and College of Biology Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, Chin)

机构地区：[1]海南大学热带农林学院海南省热带生物资源可持续利用重点实验室,海南海口570228

出　　处：《热带作物学报》2018年第3期494-501,共8页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.20163052;No.20163046)

摘　　要：细胞骨架通过结构的重排在植物响应逆境胁迫的过程中起着重要作用。本研究克隆真菌微丝结合蛋白的一段保守结构域lifeact及小鼠MAP4的微管结合结构域MBD,构建与荧光蛋白EGFP融合表达的瞬时表达载体,利用原生质体瞬时表达体系成功观察到了橡胶树叶肉细胞中微丝和微管骨架的结构,并通过对原生质体细胞进行不同处理观察胞内微丝和微管骨架的结构变化。此外,本研究还成功的捕捉到橡胶树原生质体细胞中微丝及微管的动态行为。The rearrangement of plant cytoskeleton plays important roles in response to stresses. In this study, the conserved domain lifeact of fungus ABP and MBD of mouse MAP4 was cloned, and two fluorescent protein fusion constructs with EGFP were generated. We successfully labeled the microfilaments and mierotubules in the mesophyll cell protoplasts of the rubber tree by a transient expression system, and analyzed the organizations of microfilaments and microtubules in rubber tree protoplasts under different stresses. We also traced the dynamics of visually microfilaments and microtubules in rubber tree protoplasts.

关 键 词：橡胶树 原生质体 细胞骨架 微丝 微管 逆境胁迫 

分 类 号：S794.1[农业科学—林木遗传育种]