前列腺素E_1对梗阻性黄疸大鼠肾损伤的保护作用  

Protective effect of prostaglandin E_1 on renal injury in rats with obstructive jaundice

作　　者：刘俊[1] 郑进方[1] 李灼日[1] 余成[1] LIU Jun;ZHENG Jin-fang;LI Zhuo-ri;YU Cheng(Department of Hepatobiliary Surgery, People＇s Hospital of Hainan Province, Haikou 570311, China)

机构地区：[1]海南省人民医院肝胆胰外科,海口570311

出　　处：《中国临床药理学杂志》2018年第8期961-963,967,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81260367);海南省自然科学基金资助项目(817310)

摘　　要：目的研究前列腺素E_1对梗阻性黄疸大鼠肾损伤的保护作用。方法用随机数表法将大鼠分成3组,每组15只:假手术组、模型组和实验组。用胆总管双重结扎建立大鼠梗阻性黄疸模型。手术1周后,实验组每天腹腔内注射前列腺素E10.1μg·kg^(-1),假手术组和模型组大鼠予以相同剂量0.9%Na Cl,连续注射14 d。术后第21天,处死动物,心脏采血8 m L。用免疫组织化学染色检查肾核转录因子-κB(NF-κB)、B淋巴细胞瘤-2(Bcl-2)、细胞间黏附分子-1(ICAM-1)蛋白的表达;以缺口末端标记法检测肾小管上皮凋亡指数。结果假手术组、模型组和实验组NF-κB蛋白表达分别为1.16±0.24,9.28±0.48,6.52±0.72;这3组的Bcl-2蛋白表达分别为0.86±0.54,11.28±0.27,8.36±0.48,这3组的ICAM-1蛋白表达分别为1.04±0.52,18.80±3.26,11.18±4.62;这3组的肾小管上皮细胞凋亡指数分别是0%,(5.58±0.46)%,(1.28±0.24)%。模型组与假手术组比较,差异均有统计学意义(均P<0.05);实验组的上述蛋白表达和凋亡指数与模型组比较,差异均有统计学意义(均P<0.05)。结论前列腺素E_1对梗阻性黄疸大鼠肾损伤具有保护作用,其机制可能是通过抑制NF-κB的活化和降低炎性因子的表达来实现的。Objective To investigate the protective effect of prostaglandin E1 on renal injury in rats with obstructive jaundice. Methods The Wistar rats were randomly divided into three groups（ n = 15） ： sham operation group,model group and experimental group. The rat model of obstructive jaundice was established by double ligation of common bile duct. The experimental group rats were injected intraperitoneally with prostaglandin E10. 1 μg·kg-（-1） on the seventh postoperative day and treated for 14 d. The other two groups were given the same dose of normal saline. The expression of nuclear factor-κB（ NF-κB）,B lymphocyto tumor-2（ Bcl-2） and intercellular adhesion molecule-1（ ICAM-1）protein were detected by immunohistochemistry. The tubular epithelial apoptosis index was detected by Td T-mediated d UTP nick-end labeling method. Results The expression of NF-κB protein in sham operation group,model group and experimental group were 1. 16 ± 0. 24,9. 28 ± 0. 48 and 6. 52 ± 0. 72; the expression of Bcl-2 protein in the three groups were 0. 86 ± 0. 54,11. 28 ± 0. 27 and 8. 36 ± 0. 48; the expression of ICAM-1 protein in the three groups were 1. 04 ± 0. 52,18. 80 ± 3. 26 and 11. 18 ± 4. 62; the renal tubular epithelial cell apoptosis index in the three groups were 0,（ 5. 58 ± 0. 46） % and（ 1. 28 ± 0. 24） %. Comparison between sham operation group and model group,the expression of NF-κB,Bcl-2,ICAM-1 protein and cell apoptosis index was significant（ all P〈0. 05）; comparison between model group and experimental group,the diffrence of the factors was significant（ all P〈0. 05）. Conclusion Prostaglandin E1 had a protective effect on renal injury in rats with obstructive jaundice. The mechanism of the protective effect is related to inhibit the activation of NF-κB and decrease the expression of inflammatory factors.

关 键 词：梗阻性黄疸 前列腺素E1 炎性因子 核转录因子-ΚB 凋亡指数 

分 类 号：R977.1[医药卫生—药品]